10种中草药及4种组方对罗非鱼致病性海豚链球菌体外抑菌试验  

Studies on antibacterial effects of 10 kinds of Chinese medicinal herbs and four combinations on pathogenic Streptococcus iniae from tilapia in Vitro

作　　者：张彬 黄婷 陈福艳 陈明 梁万文 

机构地区：[1]广西壮族自治区水产研究所,广西水产遗传育种与健康养殖重点实验室,南宁530021

出　　处：《淡水渔业》2011年第5期35-39,共5页Freshwater Fisheries

基　　金：国家科技支撑计划课题(2008BADB9B04);广西科学研究与技术开发计划项目(桂科基0731036)

摘　　要：采用琼脂扩散法(打孔法)研究了黄连等10种中草药及4种组方对尼罗罗非鱼(Oreochromis niloticus)致病性海豚链球菌(Streptococcus iniae)的抑制作用,并通过二倍稀释法测定抑菌作用较强的中草药对海豚链球菌的抑菌和杀菌浓度。结果显示:10种中草药对海豚链球菌均有不同程度的抑制作用,其中黄连、黄芩、大黄和黄柏的抑菌作用较强,而以黄连的抑菌效果最佳,抑菌圈平均直径达(25.67±0.58)mm。黄柏与黄连、大黄与黄芩联用抗菌作用增强,大黄与黄连联用不变,而黄柏与黄芩、黄柏与大黄、黄芩与黄连联用抑菌能力降低。The antibacterial effects of 10 Chinese herbal medicines including Coptidis rhizome, Rheum offcinale, Radix scutellariae, Phellodendron chinense, etc. to Streptococcus iniae were detected through disc agar diffusion method （punch method）, and the minimal inhibitory concentration and the minimum bactericidal concentration of four kind Chinese herbs which had significant inhibitory effects to S. iniae were measured by microdilution procedure. The results showed that 10 Chinese herbal medicine had inhibitory effects on S. iniae to different degree, among which four kinds of Chinese herbs including C. rhizome, R. scuteUariae , R. offcinale and P. chinense could effectively inhibit the growth of pathogenic S. iniae , furthermore the inhibitory effect of C. rhizome was the best, and the average diameter was （25.67 ± 0. 58） mm. Meanwhile, the experiment revealed that when used in combination, the antibacterial actions of 4 kinds of herb medicines were characterized as below： P. chinense with C. rhizome, R. offcinale with R. scutellariae had a enhanced effect, R. offcinale with C. rhizome remained unchanged, but P. chinense with R. scutellariae, P. chinense with R. offcinale, Radix scutellariae with Coptidis rhizome depressed.

关 键 词：尼罗罗非鱼(Oreochromis niloticus) 中草药 海豚链球菌(Streptococcus iniae) 抑菌圈 最小抑菌浓度 最小杀菌浓度 

分 类 号：S948[农业科学—水产养殖]