田间枣树愈伤组织诱导及不定芽的发生  

Callus induction and adventitious bud occurrence from Chinese jujube in the field

作　　者：徐娟[1] 王玖瑞[1] 刘孟军[2] 刘平[2] 代丽[2] 

机构地区：[1]河北农业大学林学院,河北保定071001 [2]河北农业大学中国枣研究中心,河北保定071001

出　　处：《河北农业大学学报》2011年第6期40-45,共6页Journal of Hebei Agricultural University

基　　金：国家科技攻关项目(2001BA502B09-04);河北省科技攻关项目;河北农业大学科技将帅计划;河北农业大学科学发展基金

摘　　要：以‘星光’和‘月光’2个枣树品种为试材,比较了截干高度和截口粗度、覆盖方式、二甲基亚砜(DMSO)、TDZ(N-苯基-N′-1,2,3-噻二唑-5-基脲)、AgNO3和秋水仙素对田间枝干截面愈伤组织诱导和不定芽发生的影响。结果表明:高枝干截面愈伤发生快,愈伤生长旺盛,截口粗度对截面愈伤的发生有影响,差异显著。"塑料袋+泥+塑料袋"是最佳覆盖方式,此方式枝干截面愈伤发生快,第5天即有透明的愈伤出现,愈伤发生率高达100%,出芽率最高达80%。添加2%二甲基亚砜导致出愈延迟。与水比较,4.0mg/L TDZ+2.0mg/L AgNO3对提高出愈率和出芽率并没有显著效果。添加秋水仙素后,不定芽的发生受到抑制,‘星光’和‘月光’均在0.025%秋水仙素时出现最小值,分别为45%和5%。与‘月光’比较,‘星光’枝干截面出愈快,出芽率高,可能更容易染色体诱变。Effect of stumping height and cutting diameter of branches,covering types,dimethyl sulfoxide（DMSO）,TDZ（Thidiazuron）,AgNO3 and colchicine on callus induction and adventitious occurrence from cross section of Chinese jujube in the field was investigated by using two varieties of Zizyphus jujube Mill.cv.＇Xingguang＇and ＇Yueguang＇.The results showed callus from cross section of higher branches formed early and developed quickly.Callus formation varied significantly with branch cutting diameter.＇Plastic bag＋ mud ＋ plastic bag＇ was the optimal covering type for callus induction and adventitious bud occurrence on cross section of branches,by which callus occurred so rapidly that transparent callus was visible in 5 days.Finally callus rate was as high as 100% and adventitious bud rate reached 80%.Callus formed lately when 2% DMSO was added.4.0 mg/L TDZ＋2.0 mg/L AgNO3 did not obviously accelerate callus induction and adventitious bud formation.However,adventitious bud was hindered by colchicines.0.025% colchicines caused the least rate of adventitious bud in ＇Xingguang＇and ＇Yueguang＇ and the value was separately 45% and 5%.Compared to ＇Yueguang＇,callus appeared early on branches cross section of ＇Xingguang＇ and its adventitious bud rate was high.So,the chromosome mutagenesis was more probably achieved in ＇Xingguang＇ than ＇Yueguang＇.

关 键 词：枣树 愈伤组织 不定芽 秋水仙素 

分 类 号：S722.3[农业科学—林木遗传育种]