转cry1C和cry2A不同抗虫基因水稻品种对非靶标害虫灰飞虱生物学特性的影响  

The biological effects of transgenic rice varieties with cry1C or cry2A on the non-target insect pest Laodelphax striatellus

作　　者：张岚[1,2] 林克剑[1] 李飞[2] 侯茂林[1] 

机构地区：[1]植物病虫害生物学国家重点实验室中国农业科学院植物保护研究所,北京100193 [2]南京农业大学植物保护学院,南京210095

出　　处：《植物保护》2011年第6期120-125,共6页Plant Protection

基　　金：转基因生物新品种培育科技重大专项(2009ZX08011-009B);国家重点基础研究发展计划("973")项目(2010CB126203)

摘　　要：为明确转cry1C和cry2A不同抗虫基因水稻品种对非靶标害虫灰飞虱生物学特性的影响,在室内恒温25℃条件下观察转cry1C和cry2A不同抗虫基因水稻品种对非靶标害虫灰飞虱寄主选择行为的影响,以及个体发育与种群发展情况,记录并分析各虫态的发育历期、存活率及实验种群生命表参数。结果表明,灰飞虱对转cry2A、cry1C基因水稻和对照亲本水稻‘明恢63’的定向选择栖息率反正弦转换值分别为27.5、28.5、26.5,差异不显著。灰飞虱对3种供试水稻品种的喜好程度基本一致,但在转cry1C基因水稻上的平均取食刺痕数508.3个/d显著高于转cry2A基因水稻(288.5个/d)和亲本水稻‘明恢63’(297个/d)。灰飞虱在两个转基因抗虫水稻上的若虫全历期、卵孵化率、各虫态存活率和成虫羽化率与对照亲本水稻相比均差异不显著。灰飞虱在转cry2A、cry1C基因水稻和对照亲本水稻‘明恢63’上的初羽化成虫鲜重分别为0.731、0.744mg和0.821mg,差异不显著。上述结果表明,转不同Bt抗虫基因水稻对非靶标害虫灰飞虱个体发育及种群生命参数影响不大,与对照亲本水稻相比较在统计学上差异不显著。The biological effects of transgenic rice varieties with cry1C or cry2A on the nontarget insect pest the small brown planthopper, Laodelphax striatellus, were studied. The effects of rice varieties with cry1C or cry2A on the host selection behavior of L. striatellus were observed at 25 ℃ under indoor conditions. The effects of rice varieties with cry1C or cry2A on individual development and population growth were also studied, by recording the developmental duration and survival rate of each stage and other parameters in the population life tables. The results showed no significant differences in the inhabiting ratios of the directional selection of L. striatellus on the rice varieties with cry1C or cry2A or the control variety ‘MH63’, with the inverse sine conversion values of 27.5, 28.5 and 26.5, respectively. The average number of probed wounds caused by L. striatellus on the transgenic cry1C variety （508.3/d） was significantly more than that on the transgenic cry2A variety （288.5/d） and the control variety ‘MH63’ （297.0/d）. There were no significant differences in the nymphal duration, egg hatchability, survival rate of each stage and adult emergence rate between transgenic rice varieties and the control variety. There were also no significant differences in the fresh weights of newly emerged adults among the transgenic cry2A variety （0.731 mg）, transgenic cry1C variety （0.744 mg） and the control（0.821 mg）. Statistically, no significant effects of transgenic rice varieties with cry1C or cry2A on the insect development and population growth of L striatellus were observed.

关 键 词：转基因水稻 非靶标害虫 灰飞虱 生长发育 

分 类 号：S435.112[农业科学—农业昆虫与害虫防治]