西索米星在卡那霉素链霉菌育种中的应用  

Application of Sisomycin in Breeding of Streptomyces kanamyceticus

作　　者：沈剑锋 樊伟明 

机构地区：[1]浙江震元制药有限公司,浙江绍兴312000

出　　处：《中国医药工业杂志》2011年第12期899-901,共3页Chinese Journal of Pharmaceuticals

摘　　要：卡那霉素（kanamycin，1）是临床上使用的重要抗生素，对大肠杆菌（Escherichiacoli）、克雷白杆菌（尉Pbsiella）、肠杆菌属（Cedecea）、变形杆菌（Bacillustermo）、结核杆菌（Bacillustuberculosis）和金黄色葡萄球菌（Staphylococcusaureus）等均有良好活性。1也是半合成抗生素丁胺卡那霉素的主要原料。Spores from degenerated strain Streptomyces kanamyceticus K19 with ＂pinpoint＂, which appeared like the eye of a needle on colony when cultured on slant were treated with physical mutagen and mutated colonies were selected from the plates containing sisomycin, a structural analogue of self metabolite. A stable strain S53 without pinpoint was screened and was cultivated in shake flask. The titer of kanamycin was enhanced from 5 589 μg/ml to 8 541 μg/ml in a 50 L fermenter. The average titer of S53 was 8 412 μg/ml in 5× 10^4L fermenter for 20 batches of fermentation culture, which was increased by 40 % than that of strain K19.

关 键 词：丁胺卡那霉素 西索米星 链霉菌 半合成抗生素 金黄色葡萄球菌 应用 育种 大肠杆菌 

分 类 号：Q78[生物学—分子生物学]