细胞角蛋白19、波形蛋白、血管内皮生长因子C和环氧化物酶2在Graves病和桥本甲状腺炎的表达及意义  

Expression and significance of cytokeratin-19, vimentin, vascular endothelial growth factor-C, and cyclooxygenase-2 in Graves＇ disease and Hashimoto＇s thyroiditis

作　　者：常娟[1] 江昌新[1] 王婷[1] 尹海波[1] 

机构地区：[1]天津医科大学病理学教研室,300070

出　　处：《中华内分泌代谢杂志》2011年第12期983-987,共5页Chinese Journal of Endocrinology and Metabolism

摘　　要：目的 探讨细胞角蛋白19（CK-19）、波形蛋白（vimentin）、血管内皮生长因子C（VEGF-C）和环氧化物酶2（COX-2）表达在弥漫性毒性甲状腺肿（diffuse toxic goiter,Graves病,GD）和桥本甲状腺炎（HT）发生和发展中的作用.方法 采用免疫组织化学SP法检测57例Graves病和58例桥本甲状腺炎中CK-19、波形蛋白、VEGF-C、COX-2的表达.结果 CK-19、VEGF-C、COX-2表达于甲状腺滤泡上皮细胞的胞浆,波形蛋白在间质和滤泡上皮细胞的胞浆均有表达.HT中CK-19和VEGF-C表达阳性率（86.2％、96.6％）及阳性强度均高于GD（43.9％、56.1％,均P＜0.05）,波形蛋白和COX-2表达的阳性率（100％、93.1％）近似于GD（ 100％、91.2％）,但COX-2的阳性强度明显高于GD（均P＜0.05）.CK-19阳性率在GDⅢ型（81.3％）明显高于Ⅰ型（15.8％）和Ⅱ型（40.9％）,HT的P型（100％）明显高于L型（66.7％）.VEGF-C阳性率在GDⅢ型（87.5％）明显高于Ⅰ型（36.8％）和Ⅱ型（50.0％,均P＜0.05）.结论 综合检测CK-19、波形蛋白、VEGF-C和COX-2四种免疫学指标,对探讨GD和HT发生、发展过程及预后评估具有一定的参考价值.Objective To study the roles of cytokeratin-19 （ CK-19 ）,vimentin,vascular endothelial growth factor-C（ VEGF-C ）,and cyclooxygenase-2 （ COX-2 ） played in the occurrence and development of Graves＇disease（GD） and Hashimoto＇s thyroiditis（HT）.Methods 57 cases of GD and 58 cases of HT were enrolled in our study.Immunohistochemistry using SP method was carried out for assessment of the expression of CK-19,vimentin,VEGF-C,and COX-2 in the thyroid tissues.Results CK-19,VEGF-C,and COX-2 were expressed in the cytoplasm of thyroid follicular epithelial cells.Vimentin was expressed both in the mesenchyma and in the cytoplasm of thyroid follicular epithelial cells.The positive rates and expression intensities of CK-19 and VEGF-C in HT （ 86.2%,96.6% ） were significantly higher than those in GD （ 43.9%,56.1%,all P〈0.05 ）.The expression intensities of vimentin and COX-2 in GD （ 100.0%,93.1% ） were similar to those in HT （ 100.0%,91.2 % ）,while the expression intensity of COX-2 in HT was significantly higher than that in GD（ all P〈0.05 ）.The positive rates of CK-1 9 were much higher in type Ⅲ （ 81.3% ） of GD than in type Ⅰ （ 1 5.8% ） and type Ⅱ （ 40.9% ） of GD,and also higher in type P（ 100% ） of HT than in type L（66.7% ） of HT.The positive rates of VEGF-C were much higher in type Ⅲ （ 87.5% ） of GD than in type Ⅰ （ 36.8% ） and type Ⅱ （ 50.0%,all P 〈 0.05 ） of GD.Conclusion Immunohistochemical detection of the expression of CK-19,vimentin,VEGF-C,and COX-2 may carry clinical significance in revealing the occurrence and development as well as evaluating the prognosis of Graves＇disease and Hashimoto＇s thyroiditis.

关 键 词：格雷夫斯病 桥本甲状腺炎 角蛋白19 波形蛋白 血管内皮生长因子C 环氧化物酶2 

分 类 号：R581.1[医药卫生—内分泌]