细胞间黏附分子-1靶向微泡超声造影成像评价肾移植后急性排异反应  

Ultrasound imaging of acute renal allograft rejection with microbubbles targeted to intercellular adhesion molecule-1

作　　者：纪丽景[1] 王宝平[1] 罗利红[1] 吴凤林[1] 

机构地区：[1]南方医科大学南方医院超声科,广州510515

出　　处：《中华超声影像学杂志》2011年第12期1070-1073,共4页Chinese Journal of Ultrasonography

基　　金：广东省自然科学基金（7005165）

摘　　要：目的 探讨靶向超声分子成像评价肾移植后急性排异反应的可行性.方法 采用“亲和素-生物素”桥接法构建携抗细胞间黏附分子-1（ICAM-1）靶向微泡（MBI）和携同型抗体对照微泡（MB）.10只SD大鼠行左侧肾异种移植术,术后72 h移植肾随机先后注入MBI和MB（间隔30 min）,分别于注入3 min后行移植肾超声造影检查,并测量移植肾声强度（VI）,最后进行肾组织病理及免疫组化检测.结果 移植肾在注入靶向超声微泡后可见肾区域明显灌注显影,延迟3 min显像MBI组在移植肾可见显著的超声显影增强.而MB组移植肾仅见轻度的超声显影增强,其显影强度较前者明显减弱.MBI组和MB组移植肾VI值分别为（27.0±7.4）U、（10.2±2.4）U,两者之间差异有统计学意义（F=64.744,P＜0.05）.结论应用靶向ICAM-1超声微泡和超声造影结合能有效评价大鼠肾移植急性排异.Objective To assess the feasibility of evaluation of renal allograft acute rejection in rat with contrast-enhanced ultrasound （ CEUS ） and targeted microbubbles.Methods Phospholipid microbubbles targeted to intercellular adhesion molecule -1 （ICAM-1）（MBI） and control microbubbles （MB） were created by conjugating monoclonal antibody against ICAM-1 or isotype control antibody to the lipid capsule via ＂avidin-biotin＂ bridging.Ten SD rats with acute renal allograft rejection were injected intravenous of MBI and MB in random order with a 30-min interval.After 3 min of intravenous injection of microbubbles,targeted CEUS imaging was performed in all rats.And then the video intensity （VI） was determined.Results In MBI group,a significant ultrasonic enhancement was observed,but it was not very obvious in MB group.Increment in VI value of transplant kidney in MBI group was great and it amounted to （27.0 ± 7.4）U,however,increment in VI value of in MB group was minor and it was merely （10.2 ± 2.4） U,Difference was evident in transplant kidney between of the two groups （P 〈 0.05）.Conclusions Molecular imaging of ICAM-1 with targeted CEUS can evaluate renal allograft acute rejection effectively.

关 键 词：超声检查 微气泡 肾移植 移植物排斥 胞间黏附分子1 

分 类 号：R699.2[医药卫生—泌尿科学]