HPLC测定天麻钩藤饮中天麻素和松脂醇二葡萄糖苷的含量  

Determination of gastrodia element and turpentine alcohot-biglucosid in Chinese medicine compound formulations Gastrodia and Uncaria Drink by HPLC

作　　者：司娜[1] 刘文[1] 宋信莉[1] 徐剑[1] 陈龙富[1] 

机构地区：[1]贵阳中医学院,贵州贵阳550002

出　　处：《中国民族民间医药》2011年第23期74-75,共2页Chinese Journal of Ethnomedicine and Ethnopharmacy

摘　　要：目的:建立天麻钩藤饮中天麻素和松脂醇二葡萄糖苷的含量测定方法。方法:采用Inertsil ODS(250mm×4.6mm,5μm)色谱柱,流动相乙腈(A)-0.2%磷酸水(B)进行梯度洗脱(0-20min,A:B=2:98;20-22min,A:B=2:98—A:B=11:89),流速为1.2mL/min,检测波长220nm,柱温30℃。结果:测定天麻素在19.3-193.2μg线性关系良好(r=0.9997),松脂醇二葡萄糖苷在6.12-61.2μg线性关系良好(r=0.9999),平均回收率分别为100.2%和98.5%。结论:本方法简便、准确,可同时测定天麻钩藤饮中天麻素和松脂醇二葡萄糖苷的含量。Objective： To establish an HPLC method for determination of gastrodia element and turpentine alcohot - biglucosid in Chinese medicine compound formulations Gastrodia and Uncaria Drink. Method： The chromatographic condition was as follows： In- ertsil ODS （250mm x 4. 6mm, 5μm） column was used with the mobile phase of acetonitrile （A） - 0. 2% （B） phosphate acid （0 -20min, A： B =2： 98; 20 -22min, A： B =2： 98--A： B = 11 ： 89） ; the flow rate was 1.2 mL · min^-1 , column temperature was at 30℃ and detection wavelength was at 220nm. Result： Gastrodia element and turpentine alcohot - biglucosid were linear in the range of 19. 3 - 193.21~g （ r = 0. 9997）, 6. 12 - 61.21xg （ r = 0. 9999） respectively. The average recovery of gastrodia element and turpentine alcohot - biglucosid were 100. 2%, 98. 5% respectively. Conclusion： This method was simple and accurate. It can be used in determination of gastrodia element and turpentine alcohot - biglucosid in Chinese medicine compound formulations Gastrodia and lJncaria Drink.

关 键 词：天麻钩藤饮 天麻素 松脂醇二葡萄糖苷 高效液相色谱 

分 类 号：R284.1[医药卫生—中药学]