海石竹叶片的离体培养与植株再生  

Culture in vitro and Plant Regeneration of Armeria maritima Leaves

作　　者：吴仕雅[1] 刘帆[1] 臧薇[1] 李方安[1] 

机构地区：[1]四川农业大学农学院,四川成都611130

出　　处：《安徽农业科学》2011年第35期21577-21579,共3页Journal of Anhui Agricultural Sciences

基　　金：四川农业大学本科论文培养计划项目(2010-1278)

摘　　要：[目的]研究海石竹叶片的离体培养及植株再生。[方法]以MS为基本培养基,对海石竹叶片的尖端、中部和基部3个不同部位进行了愈伤组织诱导、丛生芽和根分化的研究。[结果]以叶片尖端作为外植体诱导愈伤组织效果最佳,叶片中部次之,叶片基部最差。MS+6-BA 2.0 mg/L+NAA 0.75 mg/L培养基诱导叶片尖端愈伤组织的效果最好,MS+6-BA 4.0 mg/L+NAA 0.25 mg/L培养基诱导叶片中部和基部的效果最好;MS+6-BA 3.0 mg/L+NAA 0.10 mg/L培养基诱导丛生芽的效果最佳;MS+NAA 0.20 mg/L培养基诱导生根的效果最佳。[结论]建立了海石竹高效的离体培养及快速繁育体系,为其种苗的规模化、工厂化生产和其他后续的科学研究奠定了基础。[Objective] To investigate culture in vitro and plant regeneration of Armeria maritima leaves.[Method] With a basal medium MS,we made research into the callus induction,cluster buds and root differentiation on the tips,central and base of Armeria maritima leaves respectively.[Result] As to the callus induction,using leaves tip as the explants received the best effect,followed by the leaves central,and leaves base was the worst.The optimal medium for callus induction from leaves tip was MS＋6-BA 2.0 mg/L＋NAA 0.75 mg/L and the optimal medium for callus induction from leaves central and base was MS＋6-BA 4.0 mg/L＋NAA 0.25 mg/L.The optimal medium for cluster buds was MS＋6-BA 3.0 mg/L＋NAA 0.10 mg/L.The optimal medium for root differentiation was MS＋NAA 0.20 mg/L.[Conclusion] The high-efficiency in vitro culture and rapid regeneration system was established,which laid foundation for the scale and industrialized production and future scientific research of Armeria maritima.

关 键 词：海石竹 激素配比 离体培养 植株再生 

分 类 号：S132[农业科学—农业基础科学]