宁夏常用玉米自交系的SSR遗传分析  

Study of Genetic Diversity in Maize(Zea may L.) Inbred Lines of Ningxia by SSR Markers

作　　者：李新[1] 佘奎军[1] 罗湘宁[1] 许志斌[1] 

机构地区：[1]宁夏农林科学院农作物研究所,宁夏永宁750105

出　　处：《安徽农业科学》2011年第35期21594-21596,共3页Journal of Anhui Agricultural Sciences

基　　金：宁夏自治区自然科学基金项目(NZ0986)

摘　　要：[目的]对宁夏常用的82份玉米(Zea may L.)自交系进行遗传分析,划分自交系类群,以提高玉米育种效率。[方法]利用SSR标记中可用于遗传多样性分析的20对SSR引物对材料进行分析。[结果]82份自交系中共检测出335个等位基因,其中具有多态性的为108个,每对引物检测出3～10个多态性位点,平均为5.4个。SSR引物的多态性信息量(PIC)介于0.523 3～0.858 9,平均为0.7123。[结论]82份自交系划分为5个类群,分类结果基本与系谱来源一致。[Objective] In order to classify maize inbred genetic group and increase the breeding efficiency,the genetic variation of 82 maize inbred lines in Ningxia was analyzed.[Method] One types of molecular marker,simple sequence repeats（SSR） was assayed to determine the genetic diversity of these materials.[Result] 20 core-primers could test 335 allele sites in sample of 82 inbred lines,among them 108 were polymorphic.The average number of allele per SSR locus was 5.4 with arranging from 3 to 10.The average of polymorphic information content（PIC） was 0.712 3 with arranging from 0.523 3 to 0.858 9.[Conclusion] The inbred lines are classified into 5 groups and the results agree with the pedigree of the inbre line.

关 键 词：玉米自交系 SSR 遗传分析 

分 类 号：S513[农业科学—作物学]