桑叶多糖的提取工艺研究  

Study on the Extraction Processes of Polysaccharides from FOLIUM MORI

作　　者：丁锐[1] 

机构地区：[1]陕西理工学院生物学院,陕西汉中723001

出　　处：《安徽农业科学》2011年第35期21650-21652,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]优选桑叶多糖的最佳提取工艺。[方法]分别用热水浸提法和水提醇沉法、超声波辅助法3种方法提取桑叶多糖,以多糖的得率为指标,优选出最佳提取工艺。[结果]热水浸提法提取桑叶多糖的较优条件为温度80℃、时间1.5 h、料液比1∶40(g/ml);在此条件下,桑叶多糖得率约为11.3%。水提醇沉辅助法用浓度80%乙醇回流1.5 h,然后在80℃下水浴提取1.5 h,测定桑叶中多糖的得率为9.5%。超声辅助提取法提取桑叶多糖的较优方案为超声功率50 W,超声时间10 min,再在料液比1:40(g/ml)、提取温度80℃的条件下水浸提取1.5 h,测定桑叶中多糖的得率为11.6%。[结论]3种方法提取桑叶多糖的得率大小顺序依次为超声辅助法>热水浸提法>水提醇沉法,综合考虑成本、工作效率等因素,选择超声辅助提取法效果最好。[Objective] To optimize the extraction process of polysaccharides from FOLIUM MORI.[Method] Three different methods like hot water extraction,water extraction-ethanol precipitation and ultrasonic-assisted extraction were adopted to extract polysaccharides from FOLIUM MORI.Taking the yield of polysaccharides as index,the extraction process of polysaccharides from MORI FOLIUM was optimized.[Result] In hot-water immersion method,the optimum condition for extracting polysaccharides was solid-liquid ratio of 1：40 g/ml,extraction time of 1.5 h and extraction temperature of 80 ℃,which yielded a polysaccharides yield of 11.3%.In water extraction-ethanol precipitation method,the yield of polysaccharides was the highest at 9.5% if sample was refluxed for 1.5 h with 80% ethanol and extracted for 1.5 h at 80 ℃ in water bath.In ultrasonic-assisted method,the yield of polysaccharides was 11.6% if FOLIUM MORI was treated for 10 min with 50 W ultrasonic power before the extraction for 1.5 h at 80 ℃ with the solid-liquid ratio of 1：40 g/ml.[Conclusion] Based upon the yield of polysaccharides,3 different extraction methods followed a trend of ultrasound-assisted extraction hot water extraction water extraction-ethanol precipitation.Considering cost,work efficiency,etc.,ultrasonic-assisted extraction was showed to be the most promising method for extracting polysaccharides from FOLIUM MORI.

关 键 词：桑叶(FOLIUM MORI) 多糖 提取 

分 类 号：R284.2[医药卫生—中药学]