Changes of histology and capsular collagen in a rat shoulder immobilization model  

作　　者：LIU Yu-lei AO Ying-fang CUI Guo-qing ZHU Jing-xian 

机构地区：[1]Institute of Sports Medicine, Peking University Third Hospital, Beijing 100191, China

出　　处：《Chinese Medical Journal》2011年第23期3939-3944,共6页中华医学杂志（英文版）

摘　　要：Shoulder immobilization can induce adhesion of the joint, capsular contracture or lead to the condition of frozen shoulder. However, little is known about the histological effects of immobilization on the shoulder joint. This study aimed to explore the effect of immobility on the subscapular bursa （SSB） and the joint capsular content, including the distribution of typesⅠ and Ⅲcollagen, within an immobilized rat shoulder. Methods Forty-six Sprague-Dawley rats were randomly divided into one control group （n=6） and four immobilization groups （n=10 in each group）, in which the left shoulders were immobilized with plaster for 1,2, 3 and 4 weeks. At the end of each time point, 2 rats from each group were euthanized and shoulders prepared for serial histological observations of the glenohumeral joints, as well as picrosirius red and immunohistochemical observation of type Ⅲcollagen. Histological sections of the remaining rat shoulders were used for the immunohistochemical detection of the capsular content of types Ⅰand Ⅲcollagen. Results The hyperplastic synovium of the anterior capsule obstructed the communication between the SSB and the glenohumeral joint cavity at 2 and 3 weeks. The adhesion of the SSB appeared at 3 and 4 weeks. The quantitative and qualitative results showed that the capsular contents of types Ⅰand Ⅲcollagen progressively increased at 2, 3 and 4 weeks, and that type Ⅲcollagen was distributed extensively within the joint capsule at 2 and 3 weeks. Conclusion Immobilization of the rat shoulder induced synovial hyperplasia of the joint capsule, adhesion of the SSB and an increase of the capsular content of types Ⅰ and Ⅲcollagen.Shoulder immobilization can induce adhesion of the joint, capsular contracture or lead to the condition of frozen shoulder. However, little is known about the histological effects of immobilization on the shoulder joint. This study aimed to explore the effect of immobility on the subscapular bursa （SSB） and the joint capsular content, including the distribution of typesⅠ and Ⅲcollagen, within an immobilized rat shoulder. Methods Forty-six Sprague-Dawley rats were randomly divided into one control group （n=6） and four immobilization groups （n=10 in each group）, in which the left shoulders were immobilized with plaster for 1,2, 3 and 4 weeks. At the end of each time point, 2 rats from each group were euthanized and shoulders prepared for serial histological observations of the glenohumeral joints, as well as picrosirius red and immunohistochemical observation of type Ⅲcollagen. Histological sections of the remaining rat shoulders were used for the immunohistochemical detection of the capsular content of types Ⅰand Ⅲcollagen. Results The hyperplastic synovium of the anterior capsule obstructed the communication between the SSB and the glenohumeral joint cavity at 2 and 3 weeks. The adhesion of the SSB appeared at 3 and 4 weeks. The quantitative and qualitative results showed that the capsular contents of types Ⅰand Ⅲcollagen progressively increased at 2, 3 and 4 weeks, and that type Ⅲcollagen was distributed extensively within the joint capsule at 2 and 3 weeks. Conclusion Immobilization of the rat shoulder induced synovial hyperplasia of the joint capsule, adhesion of the SSB and an increase of the capsular content of types Ⅰ and Ⅲcollagen.

关 键 词：IMMOBILIZATION glenohumeral joint subscapular bursa frozen shoulder 

分 类 号：R687.3[医药卫生—骨科学]