应用PCR方法快速鉴定菜豆象  

Rapid Identification of Acanthoscelides obtectus with PCR Method

作　　者：林阳武[1] 陈文炳[1] 董文勇[1] 陈艳[1] 张东斌[1] 

机构地区：[1]福建出入境检验检疫局福建省检验检疫技术研究重点实验室,福建福州350001

出　　处：《福建林业科技》2011年第4期63-64,77,共3页Journal of Fujian Forestry Science and Technology

基　　金：福建省自然科学基金资助项目(2010J01058);福建出入境检验检疫局科技项目(FK2007-10)

摘　　要：采用形态学观察与PCR技术相结合的方法,针对口岸截获的可疑菜豆象(Acanthoscelides obtectus(Say))幼虫和成虫进行快速鉴定。采用异硫氰酸胍方法提取昆虫基因组DNA,并根据特异性引物,通过PCR技术从截获的可疑菜豆象幼虫和成虫中均扩增得到192 bp的特异性条带,经测序比对,扩增产物序列与菜豆象Cyt b目的基因序列完全相同。由此证实可疑样本即为菜豆象的幼虫和成虫。该方法缩短鉴定周期,6 h内即可快速准确鉴定未成熟的虫态,可为口岸检验检疫及快速通关提供参考。Morphological observation combined with PCR method were used to rapid quarantine and identification of dubious larvae and adult of Acanthoscelides obtectus from port.From the genome DNAs of the dubious samples,we amplified and sequenced a 192 bp of PCR product with an identical sequence of Acanthoscelides obtectus,which thus provided evidence for the dubious larvae and adult of Acanthoscelides obtectus from port.This method solved the difficult problem of rapid identification of immature insect state of Acanthoscelides obtectus in 6 h and should be useful for practical application in port of entry quarantine.

关 键 词：菜豆象 PCR 快速鉴定 检验检疫 

分 类 号：S763.38[农业科学—森林保护学] S41-30[农业科学—林学]