Spaceflight alters the gene expression profile of cervical cancer cells  

作　　者：Zhi-Jie Zhang Yong-Qing Tong Jia-Jia Wang Cheng Yang Guo-Hua Zhou Yue-Hui Li Ping-Li Xie Jin-Yue Hu Guan-Cheng Li 

机构地区：[1]Cancer Research Inst tute, Xiangya Schoo of Med c ne,Central South University, Changsha, Hunan 410078, P. R. China

出　　处：《Chinese Journal of Cancer》2011年第12期842-852,共11页

基　　金：supported in part by grants from the National Nature Science Foundation of China (No. 30672352);the 973 pre-major national basic research projects in China (No. CA04200)

摘　　要：Our previous study revealed that spaceflight induced biological changes in human cervical carcinoma Caski cells. Here, we report that 48A9 cells, which were subcloned from Caski cells, experienced significant growth suppression and exhibited low tumorigenic ability after spaceflight. To further understand the potential mechanism at the transcriptional level, we compared gene expression between 48A9 cells and ground control Caski cells with suppression subtractive hybridization (SSH) and reverse Northern blotting methods, and analyzed the relative gene network and molecular functions with the Ingenuity Pathways Analysis (IPA) program. We found 5 genes, SUB1, SGEF, MALAT-1, MYL6, and MT-CO2, to be up-regulated and identified 3 new cDNAs, termed B4, B5, and C4, in 48A9 cells. In addition, we also identified the two most significant gene networks to indicate the function of these genes using the IPA program. To our knowledge, our results show for the first time that spaceflight can reduce the growth of tumor cells, and we also provide a new model for oncogenesis study.Our previous study revealed that spaceflight induced biological changes in human cervical carcinoma Caski cells. Here, we report that 48A9 cells, which were subcloned from Caski cells, experienced significant growth suppression and exhibited low tumorigenic ability after spaceflight. To further understand the potential mechanism at the transcriptional level, we compared gene expression between 4BAg ceils and ground control Caski cells with suppression subtractive hybridization （SSH） and reverse Northern blotting methods, and analyzed the relative gene network and molecular functions with the Ingenuity Pathways Analysis （IPA） program. We found 5 genes, SUB1, SGEF, MALAT-1, MYL6, and MT-C02, to be upregulated and identified 3 new cDNAs, termed B4, B5, and C4, in 48A9 cells. In addition, we also identified the two most significant gene networks to indicate the function of these genes using the IPA program. To our knowledge, our results show for the first time that spaceflight can reduce the growth of tumor cells, and we also provide a new model for oncogenesis study.

关 键 词：基因表达谱 航天飞行 癌细胞 宫颈癌 NORTHERN 抑制性消减杂交 生长抑制 基因网络 

分 类 号：Q786[生物学—分子生物学] V475.2[航空宇航科学与技术—飞行器设计]