人体氢氯噻嗪血药浓度测定与药代动力学研究  

Determination of Hydrochlorothiazide in Healthy Human Plasma and Its Pharmacokinetic Study

作　　者：王蓉[1] 杨昆[2] 谭洁[3] 胡云芳[3] 程俊霖[3] 于翠霞[3] 余潜[3] 樊宏伟[3] 

机构地区：[1]南京医科大学附属南京第一医院心内科,江苏南京210006 [2]南京医科大学江宁校区药学院,江苏南京211166 [3]南京医科大学附属南京第一医院临床药理实验室,江苏南京210006

出　　处：《中国药业》2011年第23期17-18,共2页China Pharmaceuticals

摘　　要：目的建立测定人血浆中氢氯噻嗪质量浓度的液相色谱-串联质谱法。方法以磺胺甲基异唑为内标,采用Agilent ZobraxSB C18柱(100 mm×2.1 mm,3.0μm)为固定相,甲醇-水(80∶20,V/V)为流动相,流速为0.15 mL/min;质谱采用电喷雾离子源,多反应离子监测模式,氢氯噻嗪和内标的检测离子对分别为m/z 295.7→268.7和m/z 251.8→156.0。结果血浆中氢氯噻嗪质量浓度在1.070～214.4μg/L范围内与峰面积比值线性关系良好(r2=0.998 8),最低定量浓度为1.070μg/L,日内、日间精密度的RSD均小于13.9%,相对回收率均在100.5%～102.2%范围内。结论该方法具有较高的准确度,线性范围宽,方法灵敏、专一性好、操作简便,适用于氢氯噻嗪血药浓度的测定及临床药代动力学研究。Objective To determine the concentration of hydrochlorothiazide in human plasma by LC-MS/MS method.Methods Sulfamethoxazole was used internal standard.The evaluation of hydrochlorothiazide was carried out by Agilent Zorbax SB column（100 mm ×2.1 mm,3.0 μm） with the mobile phase of methanol/water（80 ∶20,V/V）,and the ions of analyte were detected by multiple ionic reaction monitoring mode（MRM）,the mass transition pairs of m/z 295.7→268.7 and m/z 251.8→156.0 were used to detect hydrochlorothiazide and IS,respectively.The flow rate was 0.15 mL/min.Results The calibration curve for plasma hydrochlorothiazide was linear in the range of 1.070-214.4 μg/L（r=0.998 8）.The detection limit of hydrochlorothiazide was 1.070 μg/L.Within-day RSD and between-day RSD were less than 13.9%,the relative recovery were all within 100.5%-102.2%.Conclusion This method is accurate,sensitive,specific and convenient,which could be used in the determination of plasma hydrochlorothiazide and its pharmacokinetic study in clinic.

关 键 词：氢氯噻嗪 液相色谱-串联质谱法 血药浓度 

分 类 号：R969.1[医药卫生—药理学] R983.1[医药卫生—药学]