西安地区人芽囊原虫感染状况调查  

Investigation of Blastocystis hominis infection in Xi'an city,Shaanxi province

作　　者：乔继英[1] 李小颖[2] 蔡娟[1] 张旭[1] 魏志超[3] 李亚青[3] 

机构地区：[1]西安交通大学医学院免疫学与病原生物学系,陕西西安710061 [2]西安交通大学医院,陕西西安710061 [3]西安交通大学医学院临床检测中心,陕西西安710061

出　　处：《热带医学杂志》2011年第11期1298-1300,共3页Journal of Tropical Medicine

基　　金：陕西省自然科学基金(2005C233)

摘　　要：目的了解西安市人芽囊原虫的感染状况。方法采集西安市社区居民、中学生、动物饲养人员、腹泻病人共781名受检者粪便标本,采用生理盐水直接涂片法及碘液染色法,典型形态使用数码显微镜摄像。结果健康人群和腹泻人群人芽囊原虫的感染率分别为1.99%(11/554)和7.05%(16/227),差异具有统计学意义(χ2=12.367,P<0.05);社区居民、中学生、动物饲养人员的感染率分别为2.20%(6/273)、1.20%(3/250)、6.45%(2/31),差异无统计学意义(χ2=4.033,P>0.05);粘液脓血便人群的感染率为12.16%(9/74),其他腹泻人群(水样便、糊便及脂肪泻)为7%(7/153),两者差异有统计学意义(χ2=4.382,P<0.05)。各年龄组间感染率差异有统计学意义(χ2=18.632,P<0.05)。观察到空泡型、颗粒型和包囊型虫体,可见出芽生殖、二分裂生殖和复分裂繁殖三种繁殖方式。结论人芽囊原虫在不同年龄、不同健康状况(健康和腹泻)及不同腹泻性状人群中的分布存在差异。空泡型、颗粒型多见,多以二分裂、出芽生殖方式繁殖。Objective To obtain the basic data of Blastocystis hominis infection in Xi＇ an city,Shaanxi province.Methods Fecal samples of a total of 781 people including district residents,middle school students,animal keepers and diarrheal patients were examined for Blastocystis hominis in Nov.-Dec.2007.Wet mount smear and iodine staining were employed to exam,and pictures were taken when necessary.Results Infection rates of Blastocystis hominis in healthy people and diarrheal patients were 1.99%（11 /554） and 7.05%（16 /227）,respectively.Chi-square tests revealed that there was significant difference between them（χ2=12.367,P〈0.05）.The infection rates in district residents,middle school students and animal keepers were 2.20%（6 /273）,1.20%（3 /250） and 6.45%（2 /31）,respectively.There were no significant differences among different groups of healthy people（χ2=4.033,P〈0.05）.The results in blood-containing mucous feces and other diarrhea samples（watery feces,unformed feces and fat diarrhea） were 12.16%（9 /74） and 7%（7 /153）,respectively,with significant difference（χ2=4.382,P〈0.05）.Furthermore,there was significant differences in different age groups（χ2=18.632,P〈0.05）.Vacuolar,granular and cyst form cells,and budding,binary fission and schizogony were observed.Conclusion There were significant differences on the distributions of Blastocystiis hominis in different age groups,in status of health（healthy and diarrheal） and in patients with different kinds of diarrhea.The vacuolar and granular forms,and binary fission and budding were often observed during investigation.

关 键 词：人芽囊原虫 生理盐水涂片法 碘液染色法 二分裂 

分 类 号：R382.9[医药卫生—医学寄生虫学]