响应面法优化超临界流体萃取枇杷叶中熊果酸的研究  

Optimization of Supercritical Fluid Extraction of Ursolic Acid from Follum Eriobotryae by Response Surface Methodology

作　　者：张慧恩[1] 张飞强[1] 王彩霞 刘青梅[1] 

机构地区：[1]浙江万里学院,浙江宁波315000 [2]宁波御坊堂生物科技有限公司,浙江宁波315000

出　　处：《中药材》2011年第10期1600-1604,共5页Journal of Chinese Medicinal Materials

基　　金：宁波市自然科学基金资助项目(2009A610139)

摘　　要：目的:研究超临界二氧化碳萃取枇杷叶中熊果酸的工艺。方法:通过单因素试验和响应面优化,用高效液相测定萃取物中熊果酸含量;建立枇杷叶中熊果酸超临界萃取的数学模型,确定最佳萃取工艺条件。结果:熊果酸超临界萃取的最佳工艺条件为:萃取温度61.6℃,萃取压力25.8 MPa,动态萃取时间40 min,在此条件下熊果酸的理论提取率为3.96 mg/g。结论:优化得到的该萃取工艺可靠。Objective ：To optimize the conditions for extracting ursolic acid from Follum eriobotryae with supereritical fluid extra- tion （SFE） . Methods：The contents of ursolie acid in the extracts were determined by HPLC. Based on single factor experiment and response surface methodology, a mathematical model for SFE of ursolic acid was built. Results ： The result showed that the optimum condition paremeters were as follows： temperature 61.6~C, extraction pressure 25.8 MPa, dynamic extraction time 40 min. Under these conditions, theoretical extraction rate of ursolie acid was 3.96 mg/g. Conclusion：The eptimal process is reliable.

关 键 词：枇杷叶 熊果酸 超临界萃取 响应面分析 

分 类 号：R284.2[医药卫生—中药学]