黄心春结球白菜游离小孢子培养体系的优化  

Techniques for Isolated Microspore Culture in Yellow Heading Spring Chinese Cabbage

作　　者：包美丽[1] 付颖[1] 郑鹏婧[1] 冯辉[1] 

机构地区：[1]沈阳农业大学园艺学院/辽宁省十字花科蔬菜遗传育种重点实验室,沈阳110161

出　　处：《沈阳农业大学学报》2011年第4期417-421,共5页Journal of Shenyang Agricultural University

基　　金：国家自然科学基金项目(31071792)

摘　　要：为解决黄心春结球白菜小孢子胚诱导难的问题,以引自韩国和日本的12个黄心春结球白菜杂交种为试材,对影响小孢子胚发生和发育的因素进行了研究。结果表明:供试品种间小孢子胚发生能力有极显著差异;不同品种对培养基中添加植物生长调节剂的反应不同;发现了花蕾低温密封预处理具有促进小孢子成胚的作用;MS培养基中添加0.1mg.L-1的NAA具有促进小孢子植株生根的作用。在优化培养条件的基础上,建立了针对黄心春结球白菜的游离小孢子高效培养体系,获得了11个黄心春结球白菜杂交种的445株小孢子植株,鉴定出362株双单倍体。Isolated microspore culture was carried out with 12 F1 yellow Chinese cabbage introduced from South Korea and Japan.Frequencies of embryo-genesis,factors influencing plantlet regeneration were studied.The results showed that genotypes had very significant effect on frequency of embryogenesis.The reaction of genotypes to growth regulators added in medium was different.Low temperature and hermetic sealing could promote the development of embryoids.The optimal medium for rooting was MS ＋0.1mg·L-1 NAA.An efficient culture system for yellow heading spring Chinese cabbage was established through the optimization of culture conditions,and totally 445 regenerated plants of 11 hybrid cultivar were obtained,in which 362 were identified as double haploid.

关 键 词：大白菜 小孢子培养 胚状体 

分 类 号：S634[农业科学—蔬菜学]