胆红素吸附剂功能基组成及其吸附特性研究  

PREPARATION AND ADSORPTION PROPERTIES OF BILIRUBIN ADSORBENTS WITH DIFFERENT LIGANDS BASED ON SEPHAROSE CL-4B

作　　者：王智[1] 陈书博[1] 徐丽[1] 谢健[1] 贾凌云[1] 

机构地区：[1]大连理工大学生命科学与技术学院,大连116024

出　　处：《离子交换与吸附》2011年第6期511-520,共10页Ion Exchange and Adsorption

基　　金：863资助项目(2008AA02Z210)

摘　　要：为了获得性能较佳的胆红素吸附剂,本研究考察了功能基种类、链长、偶联密度等因素对吸附剂吸附性能的影响。结果表明,当功能基具有足够的链长,与胆红素相似的疏水性及较高的偶联密度时,能够提高吸附剂的胆红素吸附能力。实验表明,链长21个原子、偶联73 mol/mL正丁胺的吸附剂对胆红素的吸附能力最强,在30℃、300mg/L的白蛋白结合胆红素溶液中,吸附1h即达到平衡,胆红素吸附量达到3.01mg/mL(90.3mg/g干重);较高的温度有利于其对胆红素的吸附。50mL材料对500mL实际病人血浆动态吸附2.5h,总胆红素(277.4mg/L)去除率达到55.2%,同时对血浆蛋白的吸附较低。所有结果显示该吸附剂具有很强的实际应用的潜力。A series of bilirubin adsorbents with different ligand type,length and density were prepared by activation of Sepharose CL-4B and their adsorption capacities to bilirubin were measured in static adsorption experiments.The results showed that the structure and properties of ligands,such as sufficient spacer-length,higher grafting density on the adsorbent and the similar hydrophobicity with bilirubin,were helpful to improve adsorption capacity of bilirubin.These experiments provided some ideas for designing new adsorbents.Among these adsorbents,the adsorbent with 73？mol/mL butylamine and 23-atom spacer showed the strongest adsorption ability to bilirubin in BSA-bilirubin solution,which could reach the adsorption balance at 30℃ in 1h,and got binding capacity of 3.01mg/mL gel.Moreover,higher temperature was more favorable to the adsorption of bilirubin.The in vitro hemoperfusion experiments were carried out by adding 50mL adsorbent into 500mL patient＇s plasma and 55.2% of bilirubin（277.4mg/L） was removed after 2.5h,and at the same time,adsorption for plasma proteins was low.These results indicated that the Butylamine-Sepharose adsorbents could be a potential choice for specific adsorption of bilirubin.

关 键 词：胆红素 正丁胺 琼脂糖凝胶 吸附 

分 类 号：O647.3[理学—物理化学]