红树内生真菌BYY-1中一个酚类化合物的分离鉴定与抗肿瘤活性  

Study on the Isolation Identification and Antitumor Activity of a Phenol Derivative from Mangrove Fungus BYY-1

作　　者：杜希萍[1,2] 赵保兵[3,4,5] 郑忠辉[3,4,5] 徐庆妍[3,4,5] 苏文金[1,2,3,4,5] 

机构地区：[1]集美大学生物工程学院,福建厦门361021 [2]厦门市食品生物工程技术研究中心,福建厦门361021 [3]厦门大学生命科学学院,福建厦门361005 [4]细胞生物学与肿瘤细胞工程教育部重点实验室,福建厦门361005 [5]福建省药物工程实验室,福建厦门361005

出　　处：《集美大学学报（自然科学版）》2011年第6期424-428,共5页Journal of Jimei University：Natural Science

基　　金：中央高校基本业务费资助项目(2010121092);福建省科技计划重点项目(2011N1008);集美大学博士启动基金资助项目(ZQ2011004)

摘　　要：采用硅胶柱层析、薄层层析、Sephadex LH-20柱层析从红树内生真菌BYY-1的次级代谢产物中分离到化合物B1.通过乙酰化、NMR、HR ESI-Q-TOF MS等技术鉴定了化合物B1的结构,确定其为首次从红树内生真菌中分离到的化合物.运用MTT法和流式细胞术研究了化合物B1的抗肿瘤活性,结果表明,化合物B1可显著抑制Hela细胞的增殖(IC50=3.3μg/mL),并且质量浓度为4μg/mL时,能诱导Hela细胞发生明显的凋亡.Compound B1 was obtained from the secondary metabolites of mangrove fungus BYY-1 using silica gel column chromatography,thin layer chromatography and Sephadex LH-20 column chromatography.The structure of compound B1 was elucidated by acetylation,NMR and HR ESI-Q-TOF MS analysis,and compound B1 was isolated from mangrove fungi for the first time.The antitumor activity of compound B1 was studied by the methods of MTT and flow cytometric DNA analysis.The results showed compound B1 could significantly inhibit the proliferation of Hela cells with IC50 3.3 μg/mL,and induce evident Hela cells apoptosis when compound B1 was 4 μg/mL.

关 键 词：红树 真菌 分离鉴定 抗肿瘤活性 

分 类 号：R93[医药卫生—生药学]