高产腈水解酶Alcaligenes faecalis UL44菌株的诱变筛选  

Mutation Breeding of Nitrilase High-Yielding Alcaligenes faecalis Strain UL44

作　　者：徐建明[1] 柳志强[1] 徐建妙[1] 张金峰[1] 郑裕国[1] 

机构地区：[1]浙江工业大学生物工程研究所,浙江杭州310014

出　　处：《微生物学杂志》2011年第5期45-48,共4页Journal of Microbiology

摘　　要：应用紫外-氯化锂和低能离子束复合诱变的方法,对1株本实验室筛选得到并保藏的腈水解酶产生菌Alcaligenes faecalis ZJB09133进行诱变育种,筛选到高产菌株UL44,其腈水解酶酶活达到74.6 U/g,较出发菌株酶活提高124.5%。得到的高产菌株UL44经过5次传代,其遗传稳定性良好。A nitrilase producer Alcaligenesfaecalis strain ZJB09133 screened and preserved in the lab was carried out composite mutation with UV-LiC1 and low-energy ion. A high yielding mutant UL44 was screened with the nitrilase activity as high as 74.6 U/g, increased by 124.5% higher than the starter strain. The obtained UL44 strain proclaimed genetically stable after passing five rounds of generation.

关 键 词：粪产碱杆菌 腈水解酶 亚氨基二乙腈 亚氨基二乙酸 诱变 

分 类 号：Q93-331[生物学—微生物学]