长效和短效NO发生剂体内干预疟原虫红内期重要侵袭分子转录水平的观察  

Transcription Levels of Important Invasive Molecules after Long-Acting and Short-Acting NO Donor to Interfere Plasmodium during Erythrocytic Stage in vivo

作　　者：郑丽[1] 李银燕[2] 刘军[1] 潘艳艳[1] 李莹[1] 延娟[1] 曹雅明[1] 

机构地区：[1]中国医科大学基础医学院免疫学教研室,辽宁沈阳110001 [2]中国医科大学附属第一医院超声科,辽宁沈阳110001

出　　处：《微生物学杂志》2011年第5期59-63,共5页Journal of Microbiology

基　　金：国家自然科学基金(30800962)

摘　　要：为探讨NO对疟原虫红内期侵袭相关分子MSP-1、AMA-1和RhopH complex转录水平的影响。通过雌性BALB/c小鼠腹腔感染1×106致死型约氏疟原虫P.yoelii 17XL,体内给予NO长效(NOC18)和短效(NOC5)发生剂进行干预后,纯化疟原虫成熟裂殖体,提取总RNA,通过Real-time PCR相对定量方法检测MSP-1、AMA-1和RhopH complex的转录水平。结果显示和正常感染组相比,NOC5处理后疟原虫侵入的关键分子MSP-1、AMA-1和RhopH complex的转录水平明显下降;而NOC18处理则未见这一现象。本研究结果提示NO抑制疟原虫侵袭关键分子的转录水平,进而可能下调疟原虫相应蛋白的表达,从而影响疟原虫的侵入过程。The effects of NO on the transcription levels of correlated invasive molecules MSP-1, AMA-1, and RhopH complex during Plasmodium erythrocytic stage were investigated. Female BALB/e mice were infected with 1 x 106 le- thal type Plasmodium yoelii 17XL in abdominal eavity, then the mice were treated with long-acting NO （NOC18） and short-acting NO （ NOC5 ） donors in vivo to carry out interference. Mature Plasmodinm sehizonts were purified and extracted their total RNA. The transcription levels of MSP-1, AMA-1, and RhopH complex were detected by real-time PCR relative quantitative determination. The results showed that the transcription levels of key molecules of MSP-I, AMA-1, and RhopH complex of Plasmodium invasion after treated with NOC5 decreased tangibly as compared with normal infected groups. However, the phenomenon was not seen in the groups treated with NOC18. These result indicated that NO inhibited the transcription levels of Plasmodium invasive key molecules, and proceeded to reduce the expression of Plasmodium corresponding protein, therefore, affected the Plasmodium invasive process.

关 键 词：疟原虫 NO发生剂 侵袭相关分子 转录 

分 类 号：R392[医药卫生—免疫学]