高效价冻干人用狂犬病疫苗暴露后免疫程序的研究  

The PEP Schedule of Freeze-Dried Rabies Vaccine with High Potency

作　　者：汝东宇[1] 雷连成[1] 

机构地区：[1]吉林大学畜牧兽医学院微生物与免疫学实验室,吉林长春130000

出　　处：《微生物学杂志》2011年第5期97-100,共4页Journal of Microbiology

摘　　要：初步确定高效价冻干人用狂犬病疫苗(6.0 IU/剂)暴露后免疫程序。制备高效价的冻干人用狂犬病疫苗(6.0 IU/剂),以狂犬病街毒CNX8601和BD06分别攻击小鼠和比格犬的咬肌,接种不同效价的狂犬病疫苗,以RFFIT法检测中和抗体,根据动物死亡情况,计算暴露后疫苗保护率,对不同效价的疫苗进行中和抗体测定和保护率统计分析。在以小鼠为实验动物的疫苗保护率研究中,冻干人用狂犬病疫苗(3.1 IU/剂)0/3/7/14/28免疫程序的保护率为40.6%,高效价的冻干人用狂犬病疫苗(6.0 IU/剂)0/3/14免疫程序的保护率为56.2%,中和抗体比较,P<0.05,2组间有显著性差异;在以比格犬为实验动物的保护效果研究中,冻干人用狂犬病疫苗的保护率(3.1 IU/剂)为70%;高效价的冻干人用狂犬病疫苗(6.0 IU/剂)的保护率为80%,中和抗体的比较,P>0.05,没有显著性差异。高效价冻干人用狂犬病疫苗暴露后免疫程序可初步确定为0、3、14 d免疫。The PEP （post-exposure prophylaxis ） schedule of freeze-dried rabies vaccine with high potency （HPRabV） （6.0 IU/dose） was established preliminarily. The HPRabV （6.0 IU/dose） was prepared, the BALB/c mice and Beagles were challenged with the street virus CNX8601 or BD06 at the masseter muscle, then inoculated with rabies vaccine of different potency （3.1 IU/dose or 6.0 IU/dose）. The rapid fluorescent focus inhibition test （RFFIT） was used to detect rabies virus neutralizing antibody （ RVNA ） in mouse and beagles sera. The protection efficacy against street rabies virus was calculated according to the animal＇ s death rates and carried out statistic analyses. The results showed that HPRabV （6.0 IU/dose） could induce the more vigorous production of RVNA in BALB/ c mice and beagles. The data indicated that the three-shot HPRabV could achieve a higher protection rate （survival rate 56.2% ） by 0/3/14 PEP schedule as compared with that of the RabV group （ survival rate 40.6% ） by 0/3/7/ 14/28 PEP schedule in mice, neutralizing antibody comparison, P 〈 0.05, the differences between the two groups were significant. The data also indicated that the HPRabV could achieve a survival rate （80. 0% ） vs. RabV （70.0%） by PEP in beagles, neutralizing antibody comparison, P 〉 0.05, the differences were not significant. Therefore, it is concluded that the PEP schedule of HPRabV for human use could initially decide the prophylactic vaccination at 0, 3, 14 days.

关 键 词：狂犬病毒 疫苗 免疫程序 效价 

分 类 号：Q939.4[生物学—微生物学]