肺炎支原体感染患儿外周血CD4^+CD25^+调节性T细胞的变化及其临床意义  

Changes and Clinical Significance of CD4^+ CD25^+ Regulatory T Cells in Children with Mycoplasma Pneumoniae Infection

作　　者：宋磊[1] 徐明[1] 侯静红[1] 赵小冬[1] 张瑜[1] 

机构地区：[1]南通市第一人民医院,江苏南通226001

出　　处：《标记免疫分析与临床》2011年第6期356-359,共4页Labeled Immunoassays and Clinical Medicine

摘　　要：探讨肺炎支原体(MP)感染患儿外周血CD4+CD25+调节性T细胞(Treg)频率变化及其意义。选取32例肺炎支原体感染患儿,其中肺炎支原体肺炎18例,肺炎支原体肺炎合并肺外症状14例;26名健康儿童,分离其外周血单个核细胞,以CD4、CD25单克隆抗体标记细胞后,用流式细胞仪(FACS)检测Treg细胞频率。FACS检测结果显示,MP肺炎组和MP肺炎合并肺外症状组患者的外周血CD4+CD25+Treg的频率均明显低于健康对照组(P<0.01);MP肺炎合并肺外症状组外周血CD4+CD25+Treg的频率明显低于MP肺炎组(P<0.01)。结论:儿童MP感染者外周血CD4+CD25+Treg细胞频率降低,免疫抑制功能下降,导致MP感染后免疫反应增强,在多脏器功能损害中起重要作用。To investigate the change and the clinical significance of CD4^＋CD25^＋ regulatory T cells（Treg） in children suffered from Mycoplasma pneumoniae（MP） infections,32 patients with MP infection were divided into mycoplasmap pneumoniae pneumonia（MPP） group（18 cases）,MPP with lesion of exo-lung group（14 cases）,and 26 health children were treated as normal control group.The peripheral blood mononuclear cells from patient samples were isolated,the frequency of Treg cell was determined with flow cytometry.The results showed that the frequency of Treg cells in patients with MP infection was significantly lower than that in normal control group（P〈0.01）,and the frequency of Treg cells in MPP with lesion of exo-lung group was obviously lower than that in MPP group（P〈0.01）.There are obvious reduce in frequency of CD4^＋ CD25^＋ Treg cells and the immune suppression in children with MP infection,it suggests that the abnormal frequency of Treg cells play a key role in the pathogenic mechanism of MP infection in children,especially in the multiple organ dysfunction.

关 键 词：肺炎支原体 CD4+CD25+调节性T细胞 

分 类 号：R725.6[医药卫生—儿科]