微气泡激发的声微流对细胞声孔效应的影响  

Effect of microbubble-induced microstreaming on the sonoporation

作　　者：邱媛媛[1,2] 张春兵[1,3] 屠娟[1] 章东[1] 

机构地区：[1]南京大学声学研究所近代声学教育部重点实验室,南京210093 [2]南通大学电子信息学院,南通226019 [3]江苏省中医院,南京210029

出　　处：《声学学报》2012年第1期91-96,共6页Acta Acustica

基　　金：国家科技部973计划(2011CB707900);国家自然科学基金(10974093;11174141;11011130201;81171659);江苏省自然科学基金(BE2010768);江苏省高校自然科学研究面上项目(11KJB140005);中央高校基本科研业务费专项资金(1103020402;1116020410;1112020401);江苏高校优势学科建设工程资助项目

摘　　要：理论及实验研究了微气泡激发的声微流对声孔效应的影响。实验采用低幅度(0.05～0.3MIPa)连续超声波信号照射MCF-7细胞,PEI:DNA的复合质粒和造影剂气泡的混合溶液,通过扫描电子显微镜测量细胞膜声孔效应。结果表明声孔大小随着激励声压的幅度和照射时间的增加而增大,平均孔径范围为100 nm～1.25μm。基于Marmottant微气泡振动模型的理论计算结果表明微气泡振动所产生的声微流引起的剪切力在低幅度超声引发声致穿孔作用中起着关键作用。In the present work, MCF-7 cells mixed with polyethylenimine： Deoxyribonucleic acid （DNA） complex and microbubbles were exposed to 1 MHz ultrasound with low acoustic driving pressures （0.05 - 0.3 MPa）. The sonoporation pores generated on the cell membrane were examined with scanning electron microscopy. The results show that larger sonoporation pores would be generated with the increasing acoustic pressure or longer treatment time, which could be useful for the enhancement of DNA transfection efficiency, the mean diameters of pores ranged from 100 nm to 1.25 μm. The calculated results based on Marmottant model indicate that the microstreaming-induced shear stress might be involved in the mechanisms of the low-intensity ultrasound induced sonoporation.

关 键 词：MCF-7细胞 微气泡 激发 扫描电子显微镜 超声波信号 照射时间 混合溶液 平均孔径 

分 类 号：R310[医药卫生—基础医学]