基于单叶反射光谱的转基因大麦光合特性分析  

Study of Photosynthetic Characteristics of Transgenic Barley Based on Reflectance of Single Leaf

作　　者：孙彩霞[1] 袁飞[1] 张玉兰[2] 陈振华[2] 陈利军[2] 武志杰[2] 

机构地区：[1]东北大学理学院,辽宁沈阳110004 [2]中国科学院沈阳应用生态研究所,辽宁沈阳110016

出　　处：《光谱学与光谱分析》2012年第1期204-208,共5页Spectroscopy and Spectral Analysis

基　　金：中央高校基本科研业务费专项基金项目(N090405011);中国科学院知识创新工程项目(KZCX2-EW-413);国家自然科学基金项目(40101016)资助

摘　　要：反射光谱分析可为植物叶片生理学过程、叶片表面及内部生化组分、结构特征等提供丰富信息。本文以转基因大麦及其原始栽培种为材料,基于单叶反射光谱检测研究了转基因大麦的反射光谱特征及指数的变化。研究结果表明,将不同大麦叶片反射率配对求得反射率比值曲线易于探查出不同大麦品系间反射光谱差异,且转基因大麦与传统对照差异较大的区域主要集中在650～700nm的红谷及红边位置附近,该波段的光谱反射率可能对外源基因的插入扰动反应敏感;而在550nm附近的绿峰及750nm以上近红外区的反射率基本无明显变化。反射光谱指数λRE,mND,SIPI,RRed/RGreen,PRI及NIRR800的差异性变化呈现出时间特性且取决于品系。如上述指数显著变化将意味着植物光合生理过程、功能及状态发生改变。Analysis of leaf reflectance can provide a rich source of information a bout plant physiological process,surface properties of the leaf,as well as int ernal structure.In the present paper,the changes of reflectance spectra and sp ectral indices for transgenic barley and non-transgenic control variety based o n measurements of single leaf were studied.The results showed that the ratio spe ctra that calculated by dividing one reflectance spectrum of transgenic barley b y its＇ non-transgenic counterpart spectra were better to illustrate fine diffe re nces among spectra of leaves from different barley varieties.Ratio spectra vari ed between each pairs,but for most ratio spectrum indicated the reflectance dif ferences between transgenic barley and non-transgenic counterpart were most obv i ous in the red spectrum around the chlorophyll peak and red edge（650～700 nm） an d nearly identical in the green spectrum near 550 nm and within the near infrar ed region（beyond 750 nm）.Across the entire calculated spectrum,the changes of reflectance within the region from 650 to 700 nm seem to be very sensitive t o respond to possible unintended effects of genetic modification.Six published reflectance indices,the red edge（λRE）,the modified normalized difference veg e tation index（mND）,the structure-independent pigment index（SIPI）,the ratio o f reflectance in the red and green regions of the spectrum（RRed/R Green）,the pho tochemical reflectance index（PRI） and the near-infrared reflectance at 800 nm （NIRR800） were used to make comparisons possible between species.The anal yses of changes of these indices indicated that they were highly dependent on growth st ages and barley varieties.Once indices described above have significant changes will mean that photosynthetic process,function and status of plants may be cha nged.

关 键 词：单叶 反射光谱 光谱参数 转基因大麦 光合作用 

分 类 号：O657.3[理学—分析化学] S512.3[理学—化学]