荷移分光光度法测定脑脉康片中烟酸占替诺的含量  

Content Determination of Xanthinol Nicotinate in Naomaikang Tablets by Charge Transfer Reaction-Spectrophotometry

作　　者：赵桂芝[1] ZHAO Gui-zhi(Liaoning Medical University,Liaoning Jinzhou 121000,China)

机构地区：[1]辽宁医学院,辽宁锦州121000

出　　处：《中国药房》2012年第1期75-77,共3页China Pharmacy

摘　　要：目的:测定脑脉康片中烟酸占替诺的含量。方法:通过烟酸占替诺与红紫素在乙醇与水混合溶液中发生荷移反应生成络合物,采用分光光度法测定其含量,检测波长为538nm;同时将测定结果与《中国药典》高效液相色谱法测定结果比较。结果:烟酸占替诺检测浓度范围为20～140mg·L-(1r=0.9989),表观摩尔吸光系数为2.46×103L·mol-1·cm-1,桑德尔灵敏度为0.1767μg·cm-2,平均回收率为100.1%(RSD=1.43%,n=6);2种方法测定样品中烟酸占替诺含量结果一致。结论:所建立的方法反应条件简单、方便快捷,为烟酸占替诺的含量测定提供了新方法。OBJECTIVE： To determine the content of xanthinol nicotinate. METHODS： The charge transfer reaction between xanthinol nicotinate and purpurin took place in alcohol-water. A spectrophotometry method was used with detection wavelength set at 538 nm. The results of study were compared with that by the HPLC method stated in Chinese Pharmacopeia. RESULTS ： The linear range was 20-140 mg. L-1 for xanthinol nicotinate （r= 0.998 9）. The apparent molar absorptivity of complex was 2.46×10^3 L. mol-1.cm-1, and Sandell＇s sensitivity was 0.176 7 μg.cm-2. The recovery was 100.1% （RSD=1.43%, n=6）. The results of content determination of xanthinol nicotinate by 2 kinds of method were in consistency. CONCLUSION： The method is simple, convenient and rapid, which can be new method for the content determination of xanthinol nicotinate.

关 键 词：烟酸占替诺 红紫素 荷移反应 分光光度法 含量测定 

分 类 号：R927.2[医药卫生—药学] R972