Generation of conditional knockout alleles for PRL-3  

作　　者：Hong Yan Dong Kong Xiaomei Ge Xiang Gao Xiao Han 

机构地区：[1]Key Laboratory of Human Functional Genomies of Jiangsu Province, Nanjing Medical University, Nanjing, Jiangsu 210029, China [2]Model Animal Research Center, Nanjing University, Nanjing, Jiangsu 210093, China.

出　　处：《The Journal of Biomedical Research》2011年第6期438-443,共6页生物医学研究杂志（英文版）

基　　金：supported by Grants from MOST of China (No. 2005CB522501, No.2006BAI23B00, and No.2006CB943503) to Xiang Gao

摘　　要：Phosphatase of regenerating liver-3 （PRL-3） is a member of the protein tyrosine phosphatase （PTP） superfamily and is highly expressed in cancer metastases. For better understanding of the role of PRL-3 in tumor metastasis, we applied a rapid and efficient method for generating PRL-3 floxed mice and investigated its phenotypes. A BAC retrieval strategy was applied to construct the PRL-3 conditional gene-targeting vector. Exon 4 was selected for deletion to generate a nonfunctional prematurely terminated short peptide as it will cause a frame-shift mutation. Conditional knockout PRL-3 mice were generated by using the Cre-loxP system and were validated by Southern blot and RT-PCR analysis. Further analysis revealed the phenotype characteristics of PRL-3 knockout mice and wildtype mice. In this study, we successfully constructed the PRL-3 conditional knockout mice, which will be helpful to clarify the roles of PRL-3 and the mechanisms in tumor metastasis.Phosphatase of regenerating liver-3 （PRL-3） is a member of the protein tyrosine phosphatase （PTP） superfamily and is highly expressed in cancer metastases. For better understanding of the role of PRL-3 in tumor metastasis, we applied a rapid and efficient method for generating PRL-3 floxed mice and investigated its phenotypes. A BAC retrieval strategy was applied to construct the PRL-3 conditional gene-targeting vector. Exon 4 was selected for deletion to generate a nonfunctional prematurely terminated short peptide as it will cause a frame-shift mutation. Conditional knockout PRL-3 mice were generated by using the Cre-loxP system and were validated by Southern blot and RT-PCR analysis. Further analysis revealed the phenotype characteristics of PRL-3 knockout mice and wildtype mice. In this study, we successfully constructed the PRL-3 conditional knockout mice, which will be helpful to clarify the roles of PRL-3 and the mechanisms in tumor metastasis.

关 键 词：PRL-3 conditional knockout alleles Cre recombinase tumor metastasis 

分 类 号：Q347[生物学—遗传学] Q426