高效液相色谱法同时测定小叶丁香不同部位中5种活性成分的含量  

Simultaneous Determination of Five Glycosides in Syringa pubenscens Turcz by HPLC

作　　者：刘普[1,2] 张创峰[1] 邓瑞雪[1] 赵天增[2] 尹卫平[1,2] 

机构地区：[1]河南科技大学化工与制药学院,河南洛阳300072 [2]河南省科学院天然产物重点实验室,郑州450002

出　　处：《中国药学杂志》2011年第24期1935-1938,共4页Chinese Pharmaceutical Journal

基　　金：河南省教育厅自然科学研究计划资助项目(2010B350003);河南科技大学博士科研启动基金资助项目(09001244;09001334)

摘　　要：目的建立RP-HPLC同时测定小叶丁香不同部位中5种糖苷类化合物含量的方法。方法采用Agilent Zorbax SB C18色谱柱(4.6 mm×250 mm,5μm);流动相为乙腈-磷酸二氢钾缓冲盐(pH 2.5)20∶80等度洗脱,流速1 mL.min-1,检测波长(松果菊苷,连翘酯苷B,类叶升麻苷,异类叶升麻苷)334 nm,橄榄苦苷285 nm。柱温30℃。结果松果菊苷在0.24～2.8μg,连翘酯苷B在0.32～1.6μg,类叶升麻苷在0.48～2.4μg,异类叶升麻苷0.36～1.8μg,橄榄苦苷在0.4～3.6μg与峰面积呈良好的线性关系,r分别为0.999 7,0.999 9,0.999 5,0.999 8,0.999 9;平均加样回收率(n=3)分别为99.19%,99.40%,99.45%,98.45%,99.5%;RSD为0.41%,0.79%,0.76%,1.01%,0.73%。结论本方法快速简便、灵敏、可靠,为药材小叶丁香的质量评价提供了科学依据。OBJECTIVE To establish an HPLC method for simultaneous determination of echinacoside, forsythoside B,verbascoside, isoverbascoside and oleuroprin in different parts of Syringa pubenscens. METHODS The separation was performed on an Agilent Zorbax SB C18 column （4. 6 mm ×250 mm,5 μm）,using acetonitrile and potassium dihydrogen phosphate solution （pH 2. 5 ） 20：80 as the mobile phase. The flow rate was 1.0 mL . min-1 ; the detection wavelengths were set at 334 nm （0 - 15 min） for echinacoside, forsythoside B, everbascoside and isoverbascoside, and 285 nm（ 15 -25 min） for oleuroprin. The column temperature was set at 30 ℃. RESULTS The linear ranges of echinacoside, forsythoside B, verbascoside, isoverbascoside and oleuroprin were 0. 24 - 2. 8 μg （ r = 0. 999 7） ,0. 32 - 1.6 μg （r =0.999 9） ,0.48 -2. 4μg （r =0. 999 5）,0. 36 - 1.8μg （r =0. 999 8） and 0.4-3.6 （r =0. 999 9）, respectively. The average recoveries （n = 3） were 99. 19% with RSD of 0. 41% ,99, 40% with RSD of 0. 79% ,99.45 % with RSD of 76% ,98.45%with RSD of 1.01% ,99. 5% with RSD of 0.73% ,respectively. CONCLUSION The method is simplelaccurate and can be used for quality control of Syringa pubenscens.

关 键 词：小叶丁香 高效液相色谱法 糖苷类化合物 含量测定 

分 类 号：R284[医药卫生—中药学]