抗南方根结线虫黄瓜-酸黄瓜渐渗系的筛选及鉴定  

Screening and Identification of Cucumber–Sour Cucumber Introgression Lines Resistant to the Root-knot Nematode Meloidogyne incognita

作　　者：叶德友[1,2] 钱春桃[1] 陈劲枫[1] 

机构地区：[1]南京农业大学园艺学院,作物遗传与种质创新国家重点实验室,南京210095 [2]甘肃省农业科学院蔬菜研究所,兰州730070

出　　处：《园艺学报》2011年第12期2281-2288,共8页Acta Horticulturae Sinica

基　　金：国家自然科学基金重点项目(30830079);国家自然科学基金项目(30972007,31071801);国家‘863’项目(2010AA10Z108)

摘　　要：采用温室盆栽苗期人工接种鉴定技术,通过对黄瓜—酸黄瓜种间杂交后代群体进行抗南方根结线虫鉴定、形态学观测和分子生物学鉴定,筛选出抗南方根结线虫的黄瓜—酸黄瓜渐渗系ILs-10-1。抗性鉴定结果表明,渐渗系ILs-10-1对南方根结线虫的抗性表现稳定;田间农艺性状和形态学观测发现,其具有小叶、短果、棕色瘤刺、多分枝的特点,性状表现介于双亲之间;SSR分子标记分析结果表明,从302对SSR引物中筛选出的1对引物SSR18648,能够在渐渗系ILs-10-1中稳定重复地扩增出野生亲本酸黄瓜的特异DNA片段,从分子水平上证实了渐渗系ILs-10-1为野生黄瓜与栽培黄瓜发生渐渗杂交的种间杂交后代。With seedling inoculation in greenhouse,progenies from interspecific cross between cultivated cucumber and sour cucumber were screened and an introgression line ILs-10-1 was identified with resistance to the root-knot nematode Meloidogyne incognita following resistance evaluation,morphological observation and molecular characterization.The results showed that the ILs-10-1 displayed stable resistance to M.incognita.In morphology,ILs-10-1 was between its parents and characterized with smaller leaf,short fruit,brown spine,multi-branching.One pair（SSR18648）out of 302 pairs of SSR primers could repeatedly amplify special DNA fragment from its wild parent of sour cucumber,confirming its nature of interspecific progenies from cross between cultivated cucumber and sour cucumber at molecular level.

关 键 词：黄瓜 渐渗系 南方根结线虫 形态学鉴定 SSR标记 

分 类 号：S642.2[农业科学—蔬菜学]