FITC染料编码流式微球阵列研究  

The study on encoding of polystyrene beads by FITC dye

作　　者：王进[1] 张瑞华[1] 兰文军[1] 

机构地区：[1]山东轻工业学院食品与生物工程学院,山东济南250353

出　　处：《山东轻工业学院学报（自然科学版）》2011年第3期17-19,29,共4页Journal of Shandong Polytechnic University

基　　金：山东省优秀中青年科学家科研奖励基金(BS2010YY044);山东省高校科研项目(J08LE12);济南市中小企业技术创新基金(2010007)

摘　　要：本文开展了FITC染料编码流式聚苯乙烯微球的研究。研究结果显示,制备的聚苯乙烯微球粒径为5.4μm,变异系数为3.04%。当低亮和高亮流式聚苯乙烯微球分别用0.4μL和125μL的FITC母液染色时间0.5 h时,可以得到微球群集中、荧光强度变异系数分别为5.28%4、.63%的二元流式微球阵列。FITC染色编码微球保存12个月,保留荧光强度为90%以上。The action of FITC to flow functional beads encoding was studied in this paper.Results from this study showed that produced microbeads had the average diameter of 5.4 μm and 3.04% CV value.Flow cytometry exhibited that the high-light and low-light bead,which constructed the duplexed bead array with 5.28%,4.63% CV value,can be successfully encoded by 0.4 μL and 125 μL FITC fluorescence solution for half an hour.Ninety percent of FITC density can be kept after 12 months.

关 键 词：聚苯乙烯微球 编码 流式细胞仪 

分 类 号：R446.61[医药卫生—诊断学]