丹参转录因子SmbHLH1基因的克隆和表达分析  

Isolation and characteristic of SmbHLH1 gene in Salvia miltiorrhiza

作　　者：汪琬宜[1,2] 蒋喜红[1,3] 张利华[1,4] 陈平[2] 申业[1] 黄璐琦[1] 

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]武汉工业学院,武汉湖北430023 [3]北京中医药大学,北京100029 [4]首都医科大学,北京100069

出　　处：《中国中药杂志》2011年第24期3416-3420,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81173490)

摘　　要：目的:获得丹参转录因子SmbHLH1全长基因,分析SmbHLH1基因在丹参不同组织部位,以及不同诱导子处理后的表达差异。方法:利用RT-PCR等技术获得SmbHLH1基因全长,利用半定量RT-PCR,分析SmbHLH1基因在丹参不同部位的表达情况,及在不同处理条件下的表达情况。结果:获得的SmbHLH1基因由999个核苷酸组成,编码332个氨基酸,蛋白相对分子质量约36 kDa;半定量RT-PCR检测,该基因在丹参的根中表达量最高,其次是茎,在叶和花中有微量表达;茉莉酸甲酯,以及酵母提取物和Ag+共同诱导,会抑制该基因的表达,水杨酸和脱落酸对该基因的影响不大。结论:丹参SmbHLH1基因是bHLH家族新成员,其功能可能与油菜素内酯信号途径有关。Objective： A novel bHLH-like gene,designated SmbHLH1,was isolated from Salvia miltiorrhiza,in order to identify a bHLH gene in related to danshinone biosysnthesis.Method： SmbHLH1 was isolated by RT-PCR,and Semi-quantitative RT-PCR was used to detect the gene expression level.Result： The full length of SmbHLH1 cDNA has an open reading frame of 999 bp.The deduced amino acid sequence of SmbHLH1 has 332 amino acid residues which forms a 36 kDa polypeptide with a calculated pI of 5.4.SmbHLH1 gene was expressed at high level in root,but low level in stem,leaf and flower of S.miltiorrhiza.The transcripts of SmbHLH1 was suppressed when the plants were treated with exogenous MeJA,Yeast＋Ag＋.The transcripts of SmbHLH1 constitutively accumulated in response to exogenous ABA and low concentration of salicylic acid.Conclusion： SmbHLH is a new member of the S.miltiorrhiza bHLH family,and its possible roles in brassinosteriods signaling responses.

关 键 词：丹参 SmbHLH1基因 克隆 表达分析 

分 类 号：S567.53[农业科学—中草药栽培]