四神丸对实验性结肠炎结肠组织Bax/Bcl-2 mRNA,Fas/FasL的调控作用  

Regulation of Sishen Wan on Bax/Bcl-2 mRNA,Fas/FasL in colonic tissue from rats with colitis

作　　者：刘端勇[1] 黄小英[1] 程绍民[1] 童文婷[1] 万盼婷[1] 管咏梅[2] 赵海梅[1] 

机构地区：[1]江西中医学院,江西南昌330025 [2]江西中医学院现代中药制剂教育部重点实验室,江西南昌330004

出　　处：《中国中药杂志》2011年第24期3484-3488,共5页China Journal of Chinese Materia Medica

基　　金：国家"重大新药创制"科技重大专项(2008ZX09310-005);江西省教育厅科学技术研究项目(GJJ09285);江西省卫生厅中医科研计划项目(2009A005;2009A101);江西省卫生厅科技计划(20092050)

摘　　要：目的:评价四神丸灌肠给药治疗实验性结肠炎疗效的同时,探讨其对结肠炎结肠黏膜Fas/FasL,Bax/Bcl-2 mR-NA的调控作用。方法:三硝基苯磺酸(trinitrobenzene sulfonic acid,TNBS)/乙醇法复制实验性结肠炎,实验动物随机分成正常组、模型组、四神丸高、中、低剂量组(10,5,2.5 g.kg-1)及柳氮磺吡啶(salicylazosulfapyridine,SASP,30 mg.kg-1)组,灌肠给药10 d后,通过体重、结肠湿重、结肠质量指数、病理损伤评分及形态学分析评价其疗效,同时分别采用流式细胞术和RT-PCT技术检测大鼠结肠组织Fas/FasL水平和Bax/Bcl-2 mRNA表达。结果:与模型组比较,四神丸中剂量组可明显降低结肠湿重及结肠指数(P<0.05),且3个剂量组均可明显降低病理损伤评分(P<0.05),同时典型治疗组病理切片分析可见上皮修复,黏膜下纤维结缔组织增生,溃疡浅而小,少量淋巴滤泡增生和炎细胞浸润,而且3个剂量组均可显著降低Fas在结肠黏膜表达(P<0.05),升高Bcl-2/Bax(P<0.05)。结论:四神丸灌肠给药有效治疗溃疡性结肠炎的作用机制之一可能是通过调控结肠组织Fas/FasL,Bax/Bcl-2 mRNA表达,延缓结肠上皮细胞凋亡或促进炎细胞凋亡实现的。Objective： To evaluate therapeutic effect of Sishen Wan on experimental colitis,and explore its mechanism by expression of Bax/Bcl-2 mRNA,Fas/FasL in colonic tissue.Method： Experimental colitis was induced by rectal administration of trinitrobenzene sulfonic acid（TNBS） dissolved in ethanol.The model animals were divided into four groups： the induced colitis but un-treated group,the induced colitis groups treated with the high,middle,low dose of Sishen Wan,and the induced colitis group treated with salicylazosulfapyridine（SASP）.After 10 day administration,the body weight,colonic wet weight,colonic weight index,colonic damage score and pathological change were evaluated,and the level of Fas and FasL by flow cytometry,Bax mRNA and Bcl-2 mRNA by reverse transcription polymerase chain reaction（RT-PCT）.Result： Compared with the model group,the colonic wet weight and colonic weight index were remarkably decreased in the middle dose of Sishen Wan group（P0.05）.The colonic injury scores were significantly reduced after rats were treated with the three doses of Sishen Wan（P0.05）.Representative restored features were observed including fewer inflammatory cellular infiltration and follicular hyperplasia,superficial and little ulcer with fibroplasia in colonic mucosa from the treated groups.The expression of Fas in the colonic mucosa was obviously down-regulated（P0.05） and the ratio of Bcl-2 mRNA/Bax mRNA was significantly up-regulated（P0.05） in the groups treated with the three doses of Sishen Wan.Conclusion： Sishen Wan might postpone colonic epithelium apoptosis or improve inflammatory cell apoptosis by regulating the expression of Fas/FasL and Bax/Bcl-2 mRNA in colonic tissue,which is possible potential path to effectively treat experimental colitis by enema.

关 键 词：四神丸 结肠炎 疗效 Fas FasL Bax MRNA Bcl-2 MRNA 

分 类 号：R285[医药卫生—中药学]