术后疲劳综合征大鼠骨骼肌能量代谢特点及人参皂苷Rb_1的干预研究  

Study on characteristics of energy metabolism in skeletal muscle of rats with postoperative fatigue syndrome and interventional effect of ginsenoside Rb_1

作　　者：谈善军[1] 周锋[1] 余震[1] 杜璐迪[1] 叶星照[1] 章晓东[1] 董千铜[1] 张波[1] 胡礼[1] 

机构地区：[1]温州医学院附属第一医院胃肠外科,浙江温州325000

出　　处：《中国中药杂志》2011年第24期3489-3493,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81070702;81171857);浙江省医学扶植重点建设学科计划(07-F03);浙江省中医药科学研究基金计划(2011ZB087);浙江省医药卫生平台重点计划(2011ZDA018);2011年浙江省大学生科技创新活动计划(新苗人才计划)项目(2001R413046)

摘　　要：目的:研究术后疲劳综合征大鼠骨骼肌能量代谢特点,并探讨人参皂苷Rb1的干预作用。方法:采用70%中段小肠切除端端吻合术作为术后疲劳综合征大鼠模型,将96只SPF级成年雄性SD大鼠按体重随机分为对照组、模型组和人参皂苷Rb1干预组。每组再按不同时间点分为术后第1,3,7,10天4小组,共12小组,每小组8只。对照组和模型组分别于术前1 h及术后每天腹腔注射1次10 mL.kg-1的生理盐水。人参皂苷Rb1干预组在相同条件下腹腔注射10 mg.kg-1的人参皂苷Rb1。分别于术后第1,3,7,10天取大鼠骨骼肌组织,用高效液相检测ATP,ADP,AMP含量,比色法检测Na+-K+-ATP酶和Ca2+-ATP酶活性。结果:与对照组相比,模型组骨骼肌ATP含量在术后第3天明显下降(P<0.05),ADP含量在术后第7,10天有不同程度的升高(P<0.05),Na+-K+-ATP酶活性在术后第3,7天有不同程度的减弱(P<0.05),Ca2+-ATP酶活性在术后第7天明显减弱(P<0.05);补充人参皂苷Rb1后骨骼肌上述时间点的各项指标均有明显改善(P<0.05),与对照组相比无明显差异。结论:术后一定时间内,术后疲劳综合征大鼠骨骼肌存在着能量代谢不足,补充人参皂苷Rb1能增强骨骼肌的能量代谢。Objective： To study characteristics of energy metabolism in the skeletal muscle of rats with postoperative fatigue syndrome（POFS） and the interventional effect of ginsenoside Rb1.Method： We chose resection of 70% of the ＂middle＂ small intestine as the rat model for POFS.Ninety-six adult male SPF SD rats were randomly divided into the control group,the model group,and the ginsenoside Rb1-treated group by body weight.And then,each group was further randomly divided into four subgroups,according to different postoperative investigated time points,such as postoperative day 1,postoperative day 3,postoperative day 7 and postoperative day 10.So the animals were divided into twelve subgroups（n=8 in each subgroup）.Rats of the control group and the model group were injected intraperitoneally with saline at the dose of 10 mL·kg-1 one hour before the operation and once a day during the postoperative days.Rats of the ginsenoside Rb1-treated group were administered 10 mg·kg-1 ginsenoside Rb1 by the same method.The skeletal muscles were sampled on postoperative day 1,3,7 and 10.The contents of ATP,ADP,AMP in skeletal muscles were determined by HPLC,and the activities of Na＋-K＋-ATPase and Ca2＋-ATPase were investigated by colorimetry.Result： Compared with the control group,the content of ATP in skeletal muscle of rats of the model group decreased significantly on postoperative day 3（P0.05）,while the content of ADP significantly increased on postoperative day 7 and 10（P0.05）.The activity of Na＋-K＋-ATPase decreased on postoperative day 3 and 7（P0.05）,and the activity of Ca2＋-ATPase decreased on postoperative day 7.After supplement of ginsenoside Rb1,on the investigated time points,all the negative changes of the indicators discovered above were significantly adjusted（P0.05） in rats of the ginsenoside Rb1-treated group,while no significant differences were investigated.Conclusion： During a certain period of postoperative time,the activity of energy metabolism is depressed in the skel

关 键 词：术后疲劳综合征 人参皂苷RB1 能量代谢 ATP ADP ATP酶 

分 类 号：R285.5[医药卫生—中药学]