经前舒颗粒对经前期综合征肝气郁证大鼠海马和下丘脑5-HT_(1A)受体表达的影响  

Effects of Jingqianshu granule on expression of 5-HT_(1A)R of PMS model rats with Liver-qi stagnation

作　　者：苏云祥[1] 薛玲[1] 

机构地区：[1]山东中医药大学中医药经典理论教育部重点实验室,山东济南250355

出　　处：《中国中药杂志》2011年第24期3498-3501,共4页China Journal of Chinese Materia Medica

基　　金：国家重点基础研究发展计划(973)项目(2011CB505102);国家自然科学基金重点项目(30930110);国家自然科学基金面上项目(30973688);山东省科技发展计划项目(2010GSF10290)

摘　　要：目的:研究经前舒颗粒对经前期综合征(premenstrual syndrome,PMS)肝气郁证大鼠海马下丘脑5-羟色胺1A受体(serotonin receptor-1A,5-HT1AR)表达水平的影响。方法:束缚造模法复制PMS肝气郁证大鼠模型,旷场实验评价模型,RT-PCR和Western blot方法分别检测大鼠海马和下丘脑5-HT1A R的mRNA及蛋白的表达。结果:经前舒颗粒给药后大鼠旷场实验得分显著升高,海马、下丘脑5-HT1AR mRNA和蛋白表达均显著升高。结论:经前舒颗粒可上调海马和下丘脑中5-HT1AR的表达,这可能是其治疗PMS肝气郁证的作用机制之一。Objective： To research the effect of the Jingqianshu granule（JQS）on the expression of serotonin receptor-1A（5-HT1AR）in hippocampus and hypothalamus of premenstrual syndrome（PMS） model rats with Liver-qi depression.Method： The PMS model rats with Liver-qi depression were induced by bandaging the limbs.The model rats were treated with JQS,and evaluated by open-field test.The expression of 5-HT1AR in hippocampus and hypothalamus was analysed by the method of RT-PCR and Western blot.Result： After the JQS were administered,the open field scores,the expression of 5-HT1AR mRNA and protein in hippocampus and hypothalamus of rats increased significantly.Conclusion： The JQS granule can up-regulate the expression of 5-HT1AR in hippocampus and hypothalamus,which maybe one of the mechanism to treat PMS with liver-qi depression.

关 键 词：经前舒颗粒 经前期综合征肝气郁证 5-HT1A受体 

分 类 号：R285[医药卫生—中药学]