土荆皮乙酸B对膀胱癌5637细胞增殖和凋亡的影响  

Effects of pseudolaric acid B on apoptosis of bladder cancer cell 5637

作　　者：曲更庆[1] 盛玉文[1] 陈波[1] 刘奔[1] 于立春[1] 

机构地区：[1]辽宁医学院附属第一医院泌尿外科,辽宁锦州121000

出　　处：《中国中药杂志》2011年第24期3535-3538,共4页China Journal of Chinese Materia Medica

摘　　要：目的:研究二萜类化合物土荆皮乙酸(pseudolaric acid B)诱导膀胱癌5637细胞凋亡的作用,探讨其作用机制。方法:MTT法测定不同浓度土荆皮乙酸作用后5637细胞的生长情况;流式细胞术检测细胞周期情况;AnnexinV-FITC/PI双染流式细胞术观察土荆皮乙酸作用后细胞的凋亡情况;免疫印迹法检测survivin和caspase-3蛋白表达的变化。结果:土荆皮乙酸能诱导5637细胞凋亡,并明显抑制survivin的表达,同时对caspase-3有上调作用。结论:土荆皮乙酸在体外可抑制5637细胞增殖,诱导其发生凋亡。土荆皮乙酸诱导5637细胞发生凋亡的生物学效应可能与survivin的表达下降,caspase-3的表达上升有关。Objective： To investigate the apoptosis of bladder cancer cell 5637 induced by pseudolaric acid B in vitro and its mechanism.Method： The cell proliferation was detected by MTT assay;the cell cycle was measured by flow cytometry;the cell apoptosis was observed by flow cytometry with Annexin V-FITC/PI double staining;the expressions of survivin protein and caspase-3 protein were detected by Western blot assay.Result： It showed that pseudolaric acid B remarkably induced apoptosis of 5637 cell line.Moreover,pseudolaric acid B suppressed survivin and up-regulated caspase-3 expression.Conclusion： Pseudolaric acid B inhibits the proliferation and induces the apoptosis of 5637 cells.The molecular mechanism of pseudolaric acid B inducing the apoptosis of 5637 cells may be associated with its action of down-regulating the expression of survivin,and up-regulating the expression of caspase-3.

关 键 词：土荆皮乙酸 膀胱癌 凋亡 survivin因子 caspase-3因子 

分 类 号：R285[医药卫生—中药学]