球毛壳ND35菌株在宿主植物上的侵染定殖  

Infection and colonization of Chaetomium globosum ND35 on host plant

作　　者：米士伟[1] 戴杨[1] 刘晓光[2] 孟庆果[1] 高克祥[1] Kurt Mendgen 

机构地区：[1]山东农业大学植物保护学院,山东省农业微生物重点实验室,泰安271018 [2]江苏大学生命科学研究院,镇江212013 [3]康斯坦茨大学生物系,德国康斯坦茨D-78457

出　　处：《植物保护学报》2011年第6期493-498,共6页Journal of Plant Protection

基　　金：国家自然科学基金(31010103038,30872024,30571498);公益性行业(农业)科研专项(201003004);山东省科技发展计划(2010GNC10911)

摘　　要：为了解球毛壳Chaetomium globosum ND35菌株在宿主植物上的侵染定殖方式和途径,以毛白杨组培苗为宿主植物,借助光学显微镜、扫描电镜、透射电镜,结合免疫荧光标记技术,研究了球毛壳ND35菌株子囊孢子萌发后在毛白杨上的侵染行为及其菌丝在组培苗根部的定殖。结果显示,子囊孢子萌发后形成的菌丝,能从杨树苗根、茎部表面细胞间的缝隙侵入或在根表面形成附着胞,进而形成侵染钉直接从表皮细胞侵入,在叶部主要从气孔侵入叶片内部。侵入根部的菌丝主要定殖于表皮细胞、外皮层细胞和细胞间隙,未进入内皮层和维管束组织。Chinese white poplar plantlets from tissue culture were served as host plant in order to understand the mode and approach of infection and colonization of Chaetomium globosum ND35 on host plant.Infection of C.globosum ND35 ascospores on Chinese white poplar plantlets after germinating and colonization of C.globosum ND35 hyphae in poplar root were investigated by means of light microscopy,scanning electron microscopy,transmission electron microscopy and with immunofluorescent labeling.The results showed that hyphae of C.globosum ND35 invaded poplar root and stem from gap of epidermal surface cells,or directly penetrated epidermis by penetration peg formed from appressorium after ascospores germinating;and invaded poplar leaves mainly from stomata.Hyphae invaded root of poplar mainly colonized in the epidemic cells,the outermost cortical layer cells or intercellular spaces of cortical layer which was not found in endodermis and vascular tissues.

关 键 词：内生真菌 球毛壳ND35 生物防治 侵染定殖 毛白杨 

分 类 号：S476[农业科学—农业昆虫与害虫防治]