球孢白僵菌对亚洲玉米螟幼虫血细胞数量和包囊作用的影响  

Effect of the fungal pathogen,Beauveria bassiana on number and encapsulation of hemocytes in Ostrinia furnacalis larvae

作　　者：陶淑霞[1,2] 李玉[2] 刘家富[1] 朱殊蕊[2] 

机构地区：[1]吉林农业大学农学院,长春130118 [2]吉林农业大学食药用菌教育部工程研究中心,长春130118

出　　处：《植物保护学报》2011年第6期527-531,共5页Journal of Plant Protection

基　　金：中国博士后基金;国家科技支撑计划(2008BADA1B);吉林省科技厅项目(2007AA021506)

摘　　要：为明确亚洲玉米螟Ostrinia furnacalis幼虫对球孢白僵菌Beauveria bassiana的细胞免疫作用,研究了注射不同剂量球孢白僵菌芽生孢子后亚洲玉米螟幼虫血细胞总数的时间动态变化和活体与离体条件下血细胞对芽生孢子的包囊作用。各处理组的血细胞总数均在注射后0.25 h达到一个低谷之后上升,注射剂量为102个/虫和103个/虫芽生孢子组血细胞总数在注射后24 h仍高于正常值,注射剂量为104个/虫和105个/虫芽生孢子组血细胞总数在注射后24 h低于正常值。在活体条件下亚洲玉米螟幼虫血细胞粘附在球孢白僵菌芽生孢子表面形成包囊;在离体条件下包囊形成的速度较慢,其致密程度不如活体状态。表明亚洲玉米螟幼虫对球孢白僵菌芽生孢子具有强烈的细胞防御能力。With the objective of determining cellular defense response of Ostrinia furnacalis to Beauveria bassiana,the dynamic changes of total hemocyte count in host larvae were studied after injection with different doses of blastospores.Meanwhile,the encapsulation responses were observed for the hemocytes in vivo and in vitro.The results showed that the total hemocyte count declined to the minimum 0.25h after injection,and increased subsequently for all treatments.The total hemocyte count was higher 24h after injection with 102 or 103 blastospores per larva than that of control.However,the total hemocyte count was lower 24h after injection with 104 or 105 blastospores than that of control.For the larvae in vivo,the hemocytes adhered to the surface of B.bassiana blastospores,and cellular encapsulation occurred.The hemocytes in vivo exhibited faster and stronger encapsulation activity against blastospores compared with those in vitro.The results indicated that O.furnacalis larvae had strong cellular defense response to the entomopathogenic fungus,B.bassiana.

关 键 词：亚洲玉米螟 球孢白僵菌 昆虫免疫 血细胞 包囊作用 

分 类 号：S476.12[农业科学—农业昆虫与害虫防治]