丹参酮ⅡA对腹膜透析液诱导的腹膜间皮细胞TGF-β1、VEGF分泌及表达的影响  

The Effect of Tanshinone ⅡA on the Secretion and Expression of VEGF and TGF-β1 in Cultured Human Peritoneal Mesothelial Cells

作　　者：于立杰[1] 蒋春明[1] 张苗[1] 

机构地区：[1]南京大学医学院附属鼓楼医院肾脏内科,江苏南京210008

出　　处：《药物生物技术》2011年第6期481-484,共4页Pharmaceutical Biotechnology

基　　金：南京市重点科技发展项目(ZKX08026)

摘　　要：研究丹参酮ⅡA磺酸钠注射液对腹膜透析液(PDF)诱导的人腹膜间皮细胞(human peritoneal meso-thelial cells,HPMCs)转化生长因子β1(Transforming growth factor beta1,TGF-β1)及血管内皮生长因子(Vascularendothelial growth factor,VEGF)分泌及mRNA表达水平的影响。将体外培养的HPMCs用于实验。细胞同步后分为对照组、PDF组、丹参酮组(含100μmol/L丹参酮ⅡA的培养基)、丹参酮1组(含50μmol/L丹参酮ⅡA的PDF)、丹参酮2组(含100μmol/L ⅡA丹参酮ⅡA的PDF)5组,干预72 h后ELISA法检测上清中TGF-β1、VEGF的含量并比较。Real-time PCR(实时定量PCR)检测TGF-β1、VEGF mRNA的表达。单四唑(MTT)法检测不同浓度丹参酮ⅡA对HPMCs增殖活性的影响。PDF组上清液中TGF-β1、VEGF含量较对照组明显上调,(P<0.01);丹参酮1,2组TGF-β1、VEGF含量较PDF组显著降低,(P<0.05),差异有显著统计学意义。丹参酮1,2组间差异无统计学意义。实时定量PCR也发现TGF-β1、VEGFmRNA表达水平在PDF组最高,丹参酮1,2组TGF-β1、VEGF mRNA表达较PDF组显著降低(P<0.01),差异有显著统计学意义。丹参酮1,2组间差异无统计学意义(P>0.05)。MTT也发现不同浓度丹参酮ⅡAHPMCs的增值活性显著高于PDF组(P<0.01),丹参酮ⅡA不同浓度间差异无显著统计学意义(P>0.05)。结论:丹参酮ⅡA能显著抑制腹膜透析液诱导的HPMCsTGF-β1、VEGF的表达,而且对HPMCs的增殖活性有良好的保护作用。To investigate the effect of Tanshinone IIA on the secretion and expression of VEGF and TGF-β1 in cultured human peri- toneal mesothelial cells（HPMCs） induced by peritoneal dialysis fluid （PDF）. The HPMCs cuhured in vitro are used for experiment. After being incubated with DMEM medium for 24 hours, the cells were divided into five groups （ DMEM medium as control, PDF- group, 100 umol/L Tanshinone II group, PDF containing 50umol/L andl00 umol/L Tanshinone IIA as Tanshinone IIA 1 group and Tanshinone IIA2 group. Each group was supplemented with equal volume of DMEM cuhure medium containing 20% FCS. After 72 hours incubation the levels of VEGF and TGF-β1 in the supematant and the mRNA of VEGF and TGF-β1 were detected. The activity of HPMCs was detected by Mitochondrial dehyrogenases actvity （MTT） in different concentrations of Tanshinone IIA. The rcesulte showed that the levels of VEGF and TGF-β1 in the supernatant were significant lower than those in PDF group. Markedly decreased levels of VEGF and TGF-β1 were found in Tanshinone IIA 1 group and Tanshinone IIA 2 group as compared with those in the PDF group. No significant difference was found between Tanshinone IIA 1 group and Tanshinone IIA2 group. Higher mRNAs of VEGF and TGF-β1 were in PDF group as compared with those in Tanshinone IIA and control groups. The upregulated secretion and mRNAs of VEGF and TGF-β1 induced by PDF can be inhibited by Tanshinone IIA in cultured HPMCs.

关 键 词：转化生长因子Β1 血管内皮生长因子 腹膜透析液 丹参酮ⅡA 

分 类 号：R285.5[医药卫生—中药学]