Actinoplanes sichuanensis03-723发酵产物95-1的分离纯化及结构鉴定  

Isolation,Purification and Structure Identification of Fermentation Product 95-1 from Actinoplanes sichuanensis 03-723

作　　者：董国霞[1,2] 张玉琴[2] 王玉成[2] 贺晓波[2] 魏玉珍[2] 李秋萍[2] 刘红宇[2] 余利岩[2] 司书毅[2] 张月琴[2] 

机构地区：[1]中国食品药品检定研究院,北京100050 [2]中国医学科学院&北京协和医学院医药生物技术研究所,北京100050

出　　处：《药物生物技术》2011年第6期501-503,共3页Pharmaceutical Biotechnology

基　　金：国家科技基础条件平台建设项目(2005DKA21200);"重大新药创制"科技重大专项(No.2009ZX09301-003;2009ZX09302-004)

摘　　要：对游动放线菌(Actinoplanes sichuanensissp.nov.)03-723菌株发酵产物中的95-1成分进行分离纯化与结构鉴定。采用乙酸乙酯萃取,硅胶柱层析、半制备HPLC等方法,对获得的95-1组分进行理化性质、UV、MS、1H-NMR、13C-NMR等数据分析及活性测定。结果:从03-723发酵产物中分离得到95-1,纯度达100%。经理化性质及波谱分析,确定95-1为Nb-乙酰色胺,其对PDF酶和E.faecium19434菌株有较弱的抑制作用和抗菌活性。结论:首次从游动放线菌03-723的发酵产物中分离纯化到Nb-乙酰色胺。To isolate,purify and identify active component 95-1 extracted from fermentation broth ofActinoplanes sichuanensis 03- 723. Methods The fermentation mycelia of strain 03-723 were soaked acetone and extracted acetic ether. Column chromatography of silica gel and semi-prep HPLC were used for purification of 95-1.95-1 was identified respectively by physicochemical properties, UV, MS, 1H-NMR, 13 C-NMR analysesetc. The inhibitory activity on PDF was determined by high throughput fluorescence detection and antibacterial activity was determined by K-B method. Results 95-1 was isolated from the fermentation broth of 03-723 and its purity reached 100%. Through physico-chemical properties and spectral analyses,95-1 was identified asNb-Acetyltryptamine. It has weak inhibitory activity on PDF and antibacterial activities againstE, faecium19434. Conclusions It was the first time thatNb-Acetyltryptamine was isolated from the strain of Actinoplanes.

关 键 词：游动放线菌 菌株03-723 分离纯化 结构鉴定 Nb-乙酰色胺 

分 类 号：R978.16[医药卫生—药品]