具有抗氧化活性的绞股蓝内生真菌的分离及研究  

Isolation,Identification and Antioxidant Activity of an Endophytic Fungi from Gynostemma pentaphyllum Makino

作　　者：尚菲[1] 魏希颖[1] 刘竹[1] 马彩霞[1] 

机构地区：[1]陕西师范大学生命科学学院,陕西西安710062

出　　处：《药物生物技术》2011年第6期519-521,共3页Pharmaceutical Biotechnology

基　　金：陕西师范大学研究生培养创新基金(No.2011CXS031)

摘　　要：从药用植物绞股蓝根部分离内生真菌,筛选出抗氧化活性菌株并对其成分进行初步检测,鉴定目的菌株。采用常规方法分离绞股蓝根部内生真菌;DPPH法和Fe3+还原力测定抗氧化能力;TLC和HPLC检测内生真菌代谢产物中绞股蓝皂苷;分子生物学方法进行种属鉴定。分离得到的10株内生真菌中G4菌株具有一定的抗氧化活性,经检测发现G4的胞内产物中含有绞股蓝皂苷,鉴定其为柔膜菌目(Helotiales)。Gynostemma pentaphyllum Makino was a well known edible and medicinal plant in oriental countries and its dominant active constituents are mainly Gypenosides（GP）. The objective is to select and identify endophytic fungus from Gynostemma pentaphyllum Makino which displays antioxidant activity and produces Gypenosides（GP） as its host. A total of 10 strains of endophytic fungi were isolated from the root of healthy, living, and symptomless tissues of Gynostemma pentaphyllum Makino, and the extracts from one strain（G4） showing antioxidant activity and had the same Rf value in TLC as that of the authentic Gypenosides（GP） and its extracts had a retention time identical with that of authentic Gypenosides（GP） in HPLC. So the strain G4 of endophytic fungus appeared to produce the same components as its host, and rDNA sequence analysis proved that CA was identified to be Helotiales.

关 键 词：绞股蓝 内生真菌 抗氧化 绞股蓝皂苷 

分 类 号：S567.237[农业科学—中草药栽培]