锰染毒对大鼠肾上腺嗜铬瘤细胞活性氧生成和miR-133b表达的影响  

Effect of Manganese Chloride on Generation of Reactive Oxygen Species and Expression of miR-133b in Pheochro-mocytoma Cells

作　　者：蔡凤霞[1] 钟伟艺[1] 张桂梅[1] 汤燕萍[1] 尤君毅[1] 吴思英[2] 李煌元[1] 

机构地区：[1]福建医科大学公共卫生学院职业与环境卫生学系环境与健康研究所毒理学与化学物安全性评价研究室,福建福州350004 [2]福建医科大学公共卫生学院流行病与卫生统计学系

出　　处：《环境与健康杂志》2011年第12期1052-1054,共3页Journal of Environment and Health

基　　金：国家自然科学基金(30800936);福建医科大学大学生创新性实验计划项目(C09051)

摘　　要：目的研究锰对大鼠肾上腺嗜铬瘤细胞(PC12)活性氧(ROS)的生成和miR-133b表达的影响。方法调整PC12细胞密度约为1×106/ml,以0(对照)、300、600μmol/L MnCl2分别染毒PC12细胞72 h,采用DCFH-DA荧光探针检测ROS生成情况;以0(对照)、100、300μmol/L MnCl2分别染毒PC12细胞24 h,采用荧光定量RT-PCR检测miR-133b相对表达水平。结果与对照组比较,300、600μmol/L MnCl2染毒组PC12细胞ROS水平升高(P<0.05);300μmol/L MnCl2染毒组PC12细胞miR-133b表达水平上调,而100μmol/L MnCl2染毒组PC12细胞miR-133b表达水平未见改变(P>0.05)。结论 MnCl2可诱导PC12细胞活性氧生成增加及miR-133b的表达上调。Objective To investigate the effect of MnC12 on induction of ROS generation and miR-133b expression in PC12 cells. Methods ROS generation in PC12 cells was measured by DCFH-DA fluorescent probe,following the PC12 cells treatment with 0,300 and 600 μmol/L of MnC12 for 72 h. The relative level of miR-133b expression was measured by real time RT-PCR,following the PC12 cells treatment with 0,100 and 300 μmol/L MnC12 for 24 h. Results ROS generation in the group treated with 300 and 600 μmol/L of MnC12 were higher than compared with the vehicle control group （P〈0.05）. The relative level of miR-133h expression of PC12 cells in 300 μmol/L of MnCl2 group was higher than that of the vehicle control group （P〈O.05）. But there was no change in the relative level of miR-133b expression of PC12 cells treated with 100 μmol/L of MnC12. Conclusion MnC12 may induce ROS generation and up-regulate the expression of miR-133b in PC 12 cells.

关 键 词：锰 活性氧 miR-133b 肾上腺嗜铬瘤细胞 

分 类 号：R994.6[医药卫生—毒理学]