J亚群禽白血病病毒gp85单抗1E3株抗原识别表位的初步分析  

Preliminary analysis of avian leukosis virus subgroup J gp85 antigenic epitope recognized by monoclonal antibody 1E3

作　　者：莫虹斐[1,2] 余多[1] 孙淼[1] 陈福勇[1] 曹红[1] 

机构地区：[1]中国农业大学动物医学院,北京海淀100193 [2]西北农林科技大学动物医学院,陕西杨凌712100

出　　处：《中国兽医杂志》2011年第12期3-7,共5页Chinese Journal of Veterinary Medicine

基　　金：国家自然科学基金(U0831002)

摘　　要：J亚型禽白血病是由J亚群禽白血病病毒引起的一种以成髓细胞增生、免疫抑制、生长发育受阻为特点的传染性肿瘤疾病,于1988年首次发现,其病毒囊膜表面蛋白gp85决定了病毒亚群特异性。本试验将原核表达质粒pET-28a-J gp85中的gp85基因分段亚克隆至重组表达质粒pGEX-6p-1上并在大肠杆菌中成功地表达,用ALV-J S1gp85单克隆抗体1E3株对分段表达的gp85蛋白进行初步的免疫印迹分析。结果表明:ALV-J S1gp85单抗1E3株所识别的抗原表位位于蛋白N端的第69至112个氨基酸之间。这将为ALV-J gp85抗原决定簇所在位点及其免疫学功能的研究奠定基础。Subgroup J avian leukosis virus （ALV-J） can cause a marrow cell proliferation, immune suppression, retarded growth and tumor. In this study, the gp85 gene of avian leukosis virus subgroup J S1 strain isolated in China was sub-cloned into the expression vectors pGEX-6p-1 for constructions of expression plasmids and successfully expressed in E. coli. J-gp85 was dissected into several overlapping epitopes, which were used to react with McAbs by Western-blot. The results showed that the 69th to 112th amino acids in the gp85 protein could be further the study on epitopes located in ALV-J recognized by monoclonal antibodies 1E3. These data will gp85 and functional study of this protein.

关 键 词：J亚群禽白血病病毒 gp85蛋白 单克隆抗体 抗原表位 

分 类 号：S852.659.3[农业科学—基础兽医学]