皱纹盘鲍一种丝氨酸蛋白酶抑制因子克隆与表达分析  

Molecular Cloning,Characterization and Expression of a Serin Gene from Abalone Haliotis discus hannai Ino

作　　者：丁鉴锋[1,2,3] 张国范[1] 李莉[1] 

机构地区：[1]中国科学院青岛海洋研究所,山东青岛266071 [2]中国科学院研究生院,北京100049 [3]大连海洋大学,辽宁大连116023

出　　处：《水产科学》2011年第12期731-738,共8页Fisheries Science

基　　金："973"国家基础研究项目(2010CB126401);农业部国家公益性贝类专项(nyhyzx07-047)

摘　　要：通过RACE方法获得皱纹盘鲍丝氨酸蛋白酶抑制因子的cDNA序列。该序列全长1446个碱基,其中开放阅读框为410个碱基。该基因编码的氨基酸与丝氨酸蛋白酶抑制因子Serpin家族的结构相近。另外,在基因启动子区发现一些与免疫和癌症发生有关的转录因子结合位点。采用qRT-PCR方法分析该基因不同组织中表达情况,结果发现,在所有检测的组织包括外套膜、鳃、消化腺、血淋巴和肌肉中均有表达。另外,使用鳗弧菌感染并检测鳃组织中该基因表达情况,结果发现,细菌刺激后该基因表达量逐渐升高,表明丝氨酸蛋白酶抑制剂基因在皱纹盘鲍抗菌反应中能够发挥免疫调节作用。The cDNA sequence of serine protease inhibitors gene was identified from abalone Haliotis discus hannai（named as HdSpi） using RACE method.The full-length cDNA of the serine protease was found to be 1446 bp with a 1230 bp ORF encoding 410 amino acids,and the structure of deduced amino acid sequence similar with the Serine family.Many putative transcription factor binding sites involved in the immune system and cancer were found in the promoter region of HdSpi.HdSpi expression was detected in all the examined tissues by Quantitative real-time（qRT-PCR）,and in an expression profile of the abalone gills challenged with bacteria Vibrio anguillarum.HdSpi transcripts were found to be expressed in mantles,gills,digestive tract,haemocytes and muscles.The increased expression of HdSpi was also observed after bacterial stimulation.These results suggest that the HdSpi have an immunomodulatory function in antibacterial response of the abalone.

关 键 词：皱纹盘鲍 丝氨酸蛋白酶抑制因子 免疫 

分 类 号：Q78[生物学—分子生物学]