内毒素与机械通气对大鼠膈肌氧化损伤的影响  

Effects of Endotoxin and Mechanical Ventilation on Diaphragmatic Oxidative Injury in Rats

作　　者：李盈[1] 杨华丽[1] 代宜凝[1] 高春娥[1] 陈列[1] 焦光宇[1] 

机构地区：[1]中国医科大学附属盛京医院呼吸内科,沈阳110004

出　　处：《中国医科大学学报》2011年第12期1085-1087,共3页Journal of China Medical University

基　　金：辽宁省教育厅高校科研计划(2008-775);教育部留学回国人员科研启动基金(教外司留2008-890)

摘　　要：目的诱导型一氧化氮合酶(iNOS)是氧化应激的核心指标。本研究通过比较iNOS在脓毒症(sepsis)与机械通气(CMV)大鼠膈肌中iNOS的变化,分析sepsis及CMV对膈肌氧化应激的影响。方法将32只健康SD大鼠随机分为4组(n=8),对照组s、epsis组、CMV组s、epsis+CMV组。采用HE染色在光镜下观察肺组织的病理改变,采用免疫组化分析膈肌组织中iNOS的表达。结果 sepsis组肺组织病理发生改变,而sepsis+CMV组肺部病理改变有所减轻。sepsis+CMV组肺间质水肿明显减轻,中性粒细胞及白细胞渗出减少。免疫组化显示iNOS的表达在sepsis组、CMV组较对照组有明显增加(P<0.05)s;epsis+CMV组iNOS表达较单独sepsis组及CMV组增加更为明显(P<0.05)。结论内毒素8 mg/kg腹腔注射24 h,可明显增加膈肌的氧化损伤;CMV 8 h也可以导致膈肌氧化损伤增强;内毒素联合CMV比单独sepsis组及CMV组膈肌氧化损伤增加更明显,这可能是呼吸衰竭及撤机困难的重要原因。Objective Inducible NO synthase（iNOS） is the core indicator of oxidative stress.To investigate the oxidative injury in rat diaphragm,we compared the changes of iNOS in rat diaphragm in sepsis and controlled mechanical ventilation（CMV） animal models.Methods 32 Sprague Dawley（SD） rats were alocated randomly into four groups（n =8 in each group）：control group,sepsis group,CMV group and sepsisv＋CMV group.HE staining was used in the pathologic analysis of lung tissue;immunohistochemistry method was used in detection of iNOS expression in diaphragm.Results Pathologic changes of lung tissue were found in sepsis group.However,pathologic changes were relieved in sepsis＋CMV group.The iNOS expression in sepsis group and CMV group were increased remarkably compared to control group（P 0.05）,and that in sepsis＋CMV group was increased compared to sepsis group or CMV group respectively（P 0.05）.Conclusion Oxidative injury was increased in diaphragm 24 h after intraperitoneal injection of endotoxin（8 mg/kg i.p） or 8 h after CMV.Combination of endotoxin and CMV could result in more oxidative stress than one factor in diaphragm.This may be an important reason for respiratory failure and failure to weaning.

关 键 词：氧化损伤 内毒素 机械通气 膈肌 

分 类 号：R565[医药卫生—呼吸系统]