大鼠局灶性脑挫伤后Homer蛋白表达变化  

Homer Protein Expression after Focal Brain Contusion in Rats

作　　者：王福远[1,2] 李如波[1] 梁红霞[1] 沈宇 郭晓冲[1] 王正印[1] 

机构地区：[1]中国医科大学法医学院法医病理学教研室,辽宁沈阳110001 [2]宁波市公安局鄞州分局,浙江宁波315100 [3]苍南县公安局刑事技术大队,浙江温州325800

出　　处：《法医学杂志》2011年第6期409-412,共4页Journal of Forensic Medicine

基　　金：辽宁省高等学校科研项目(2008828)

摘　　要：目的观察局灶性脑挫伤后Homer蛋白表达的变化规律,探讨其表达变化与损伤时间的相关性。方法建立大鼠钝力性局灶性脑挫伤模型,应用免疫组织化学技术和Western印迹法观察脑损伤后不同时间脑组织中Homer蛋白表达的变化。结果对照组、假手术组及伤后0.5h组脑组织中有少量Homer蛋白染色阳性细胞,伤后3 h组脑组织Homer蛋白染色阳性细胞增多,伤后12 h组阳性细胞数达到高峰,伤后1 d起阳性细胞数持续下降,至伤后7 d降到基础水平。Homer蛋白染色及Western印迹法显示,Homer蛋白表达量在相邻组间差异均具有统计学意义。结论大鼠钝力性局灶性脑挫伤后损伤周边区Homer蛋白表达变化与脑挫伤时间具有相关性。Objective To investigate Homer protein expression after focal brain contusion and explore the relationship between expression and injury time.Methods Focal brain contusion in rats was established and Homer protein expression in brain at different injury intervals after contusion was detected by immunohistochemistry and Western blotting.Results A small amount of Homer positive expression cells were detected in control group,sham operated group and experimental group（0.5 h after contusion）.The amount of Homer positive expression cells increased after 3 h and reached peak 12 h after contusion.The amount of positive cells continued to decrease 1 d after contusion and to the base level 7 d after contusion.Homer protein expression based on immunohistochemistry and Western blotting had statistical difference among adjacent groups.Conclusion Expression of Homer protein near the focal contusion area shows time dependence after brain contusion in rats.

关 键 词：法医病理学 脑损伤 印迹法 蛋白质 免疫组织化学 Homer蛋白 大鼠 

分 类 号：DF795.1[医药卫生—法医学]