GC-MC法测定血液中丙泊酚  

Determination of Propofol in Human Blood by GC-MS

作　　者：孙英英[1,2] 向平[1] 刘伟[1] 卜俊[1] 沈敏[1] 

机构地区：[1]司法部司法鉴定科学技术研究所上海市法医学重点实验室,上海200063 [2]苏州大学医学部法医学系,江苏苏州215123

出　　处：《法医学杂志》2011年第6期430-433,共4页Journal of Forensic Medicine

基　　金：中央级公益性科研院所基本科研业务费专项资金项目(GY0903);上海市法医学重点实验室资助项目(11DZ2271500)

摘　　要：目的建立血液中丙泊酚的气相色谱-质谱联用（GC-MS）分析方法。方法血液以2,4-二甲基-6-叔丁基苯酚为内标,用乙醚进行液液萃取,离心后取有机层,水浴下挥干,GC-MS检测。结果血液中丙泊酚与内标分离良好,在0.02~10μg/mL范围内线性良好,线性方程为y=0.3136x-0.0068,相关系数为0.9997,日内精密度与日间精密度（RSD）均小于4.8%,检出限（LOD）为0.005μg/mL。利用该方法测得实际案例血液中丙泊酚质量浓度为0.14μg/mL。结论建立的GC-MS方法简单、灵敏、可靠,可适用于血液中丙泊酚的法医毒物分析和临床药物监测。Objective To establish a gas chromatography-mass spectrometry（GC-MS） method for determination of propofol in human blood.Methods Propofol and 2-（tert-Butyl）-4,6-dimethylphenol（internal standard） were isolated from human blood samples with liquid-liquid ether extraction.The organic layer was collected after centrifugation and dried using the water bath.The extracted residue was analyzed by GC-MS.Results Propofol and the internal standard showed a good separation with a good linear concentration ranged from 0.02 to 10 g/mL in blood.The linear function was y=0.313 6 x-0.006 8 with the correlation coefficient of 0.999 7.The precision of intra-day and inter-day were less than 4.8% and the lower limit of detection of propofol was 0.005 g/mL.Propofol concentration of blood was 0.14 g/mL using this method in the practice work.Conclusion The GC-MS method is rapid,sensitive,reliable and suitable for qualitative and quantitative analysis propofol of blood in forensic toxicological analysis and clinical drug monitoring.

关 键 词：法医毒理学 气相色谱-质谱法 丙泊酚 血液 

分 类 号：DF795.1[医药卫生—法医学]