‘霞多丽’胚性细胞悬浮系的建立及植株再生  

Establishment of the Embryogenic Cell Suspensions Cultures and Plant Regeneration of Wine Grape ‘Chardonnay’

作　　者：侯丽霞[1] 刘伟[1] 张雅丽[2] 刘新[1] 卢江[2] 

机构地区：[1]青岛农业大学生命科学学院山东省高校植物生物技术重点实验室,山东青岛266109 [2]中国农业大学食品科学与营养工程学院,北京100083

出　　处：《北方园艺》2011年第23期93-96,共4页Northern Horticulture

基　　金：农业部948计划资助项目(2006-G26)

摘　　要：以‘霞多丽’前胚物质为试材,初步建立了‘霞多丽’胚性细胞悬浮系,并研究了不同激素及其浓度配比,不同种类及浓度的碳源以及谷氨酰胺对悬浮细胞生长的影响。结果表明:2,4-D和NOA均可以抑制胚性细胞的分化,但2,4-D更有利于细胞的增殖,最佳的2,4-D附加浓度为1mg/L。同等浓度(0～60g/L)的麦芽糖比蔗糖更利于细胞的生长,15g/L为‘霞多丽’胚性悬浮细胞生长的最佳麦芽糖浓度。附加谷氨酰胺可以显著提高悬浮细胞的生长,其中以500mg/L为最佳。An efficient system for the establishment and multiplication of highly prolific embryogenic cell cultures of wine grape ‘Chardonnay’ was developed.Using pro-embryogenic masses（PEMs）as starting material,cell suspensions was initiated in liquid medium containing NOA（1.0 mg/L as growth regulator.The effects of different hormones,carbon sources with their concentrations and glutamine on multiplication of ‘Chardonnay’ embryogenic suspension cell were tested.The results indicated that the influences of each factor on multiplication were significantly different.Both 2,4-D and NOA could inhibit differentiation of‘Chardonnay’ embryogenic suspension cell.But 2,4-D was more beneficial to proliferative and 1 mg/L was the optimum concentration.Maltose was better than sucrose with the same level（0～60 g/L）,15 g/L was the best additional concentration.Glutamine was helpful to the multiplication of embryogenic suspension cell and 500 mg/L was the optimum.

关 键 词：'霞多丽’ 胚性细胞 悬浮系 激素 碳源 谷氨酰胺 

分 类 号：S663.104.3[农业科学—果树学]