农杆菌介导的几丁质酶基因和β-1,3-葡聚糖酶基因转化辣椒研究  

Study on the Transforming Chitinase and β-1,3-glucanase to Pepper Via Agrobacterium tumefaciens

作　　者：谢立波[1] 郭亚华[1] 王雪[1] 高永利[1] 周宇[1] 

机构地区：[1]黑龙江省农业科学院园艺分院,黑龙江哈尔滨150069

出　　处：《北方园艺》2011年第23期97-101,共5页Northern Horticulture

基　　金：航天育种工程资助项目(2006HT20012);黑龙江省自然基金重点资助项目(ZD200818-01);黑龙江省自然基金资助项目(C200943)

摘　　要：以苗龄7～10d的辣椒组培苗为被侵染的外植体,通过农杆菌介导几丁质酶基因和β-1,3-葡聚糖酶基因,研究基因型、外植体类型、抗生素浓度对辣椒遗传转化的影响,以期获得抗真菌病的转基因辣椒植株。结果表明:不同基因型间、外植体类型、抗生素种类和浓度对辣椒的遗传转化影响存在较大差异。以几丁质酶和β-1,3-葡聚糖酶为目的基因,应用根癌农杆菌介导法对辣椒进行了遗传转化,获得了3株抗性植株。In vtro plant of pepper at about 7～10 d were used as material of infection for transformation of fungi resistant genes Chitinase and β-1,3-glucanase via Agrobacterium tumefaciens to study the impact from genotype,explant type and concentration of antibiotic on genetic transformation of pepper,in order to obtain resistant plant through gene modification.The results showed that significant difference appeared in the transformation with various genotypes,explants types,varieties of antibiotics and the concentration.Three resistant pepper plants were gained after transformation of genes of chitinase and β-1,3-glucanase via agrobacterium tumefaciens.

关 键 词：几丁质酶基因 Β-1 3-葡聚糖酶基因 辣椒 转化 

分 类 号：S641.303.6[农业科学—蔬菜学]