反相高效液相色谱法测定癌痛消颗粒中野黄芩苷的含量  

Determination of Scutellarin in Aitongxiao Granule by RP-HPLC

作　　者：李文仕 

机构地区：[1]广西百色食品药品检验所,广西百色533000

出　　处：《中国医药导报》2011年第36期64-65,68,共3页China Medical Herald

摘　　要：目的:建立测定癌痛消颗粒中野黄芩苷含量的方法。方法:用石油醚(60～90℃)除杂质,用甲醇提取的方法处理样品,采用反相高效液相色谱法对癌痛消颗粒中野黄芩苷进行含量测定。色谱柱为Shimadzu Class-VP-ODS C18色谱柱(4.6 mm×250 mm,5μm),以甲醇-0.1%磷酸溶液(34∶66)为流动相,流速为1.0 ml/min,柱温为室温,检测波长335 nm。结果:野黄芩苷进样浓度在21.8～130.8μg/ml范围内线性关系良好(r=0.999 5),平均回收率为97.87%,RSD=1.74%(n=6)。结论:该方法操作简便,专属性强,重复性好,可作为癌痛消颗粒中野黄芩苷质量控制方法。Objective：To establish a method for the content determination of Scutellarin in Aitongxiao Granule.Methods：Petroleum ether was used（60-90℃） to remove the impurity,the method of Methanol extraction was used to dispose the sample,Scutellarin of Aitongxiao Granule was determined by RP-HPLC.The column was Shimadzu Class-VP-ODS C18（4.6 mm × 250 mm,5 μm） and the mobile phase was mixture of methanol-0.1% phosphoric acid（34∶66） at the flow rate of 1.0 ml/min.The column temperature was at room temperature and the detection wavelength was 335 nm.Results：The Scutellarin sample showed a good linear relationship in the range of 21.8-130.8 μg/ml,r=0.999 5,and the average rate of recovery was 97.87% with RSD=1.74%（n=6）.Conclusion：The method is simple,specific,and accurate with good reproducibility.It can be used for quality control of Scutellarin in Aitongxiao Granule.

关 键 词：癌痛消颗粒 反相高效液相色谱法 野黄芩苷 

分 类 号：R927.2[医药卫生—药学]