吗啡后处理对兔缺氧复氧损伤肾脏组织的保护作用  

Protective Effects of Morphine Postconditioning on Renal Tissue against Acute Hypoxic-reoxygenation Injury in Rabbit

作　　者：江晓琴[1] 倪娟[1] 杨沛[2] 黄蔚[1] 李华凤[1] 

机构地区：[1]四川大学华西第二医院麻醉科,成都610041 [2]四川大学华西第二医院妇产科,成都610041

出　　处：《四川大学学报（医学版）》2012年第1期38-40,共3页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(批准号30672025)资助

摘　　要：目的探讨吗啡后处理对兔缺氧复氧损伤肾脏组织的延迟保护作用及其机制。方法雄性新西兰大白兔30只随机分为正常对照组、缺氧复氧组(H/R组)和吗啡后处理组(H/R+MO组)。H/R组和MO+H/R组吸入8%O23h后立即分别静脉注射生理盐水5mL和吗啡3mg/kg,并空气复氧48h。比较各组肾功能变化和肾脏组织病理学改变,凋亡指数。结果与正常对照组比,H/R组和H/R+MO组复氧48h,血肌酐(SCr)和尿素氮(BUN)水平提高(P均<0.05),肾小管上皮细胞凋亡指数升高(P<0.05);与H/R组相比,H/R+MO组缺氧复氧48h,SCr和BUN水平降低,肾小管上皮细胞凋亡指数降低(P均<0.05)。结论吗啡后处理可能减轻缺氧复氧损伤引起的肾小管上皮细胞的凋亡,降低SCr和BUN水平,发挥肾脏保护作用。Objective To investigate the protective effects of morphine postconditioning on the renal hypoxic/reoxygenation injury in rabbit and the mechanisms involved.Methods Hypoxic/reoxygenation injury was induced with inhalation 8% O2 3 hours followed by breathing in air 48 hours.Thirty male white New Zealand rabbits were randomly divided into 3 groups： normal control group,hypoxic/reoxgenation group（H/R group） and morphine hypoxic postconditioning group（MO＋H/R group）.Animals in H/R and MO＋H/R group received 5 mL of saline or morphine 3 mg/kg respectively before induction reoxygenation.The levels of serum creatinine（SCr） and blood urea nitrogen（BUN） were detected respectively at 48 hour after hypoxic and reoxgenation happened.Pathological changes in the different groups were also evaluated by light microscope.Renal apoptosis was determined quantitatively by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling（TUNEL）methods.Result The SCr and BUN in H/R group and MO＋H/R group were much higher respectively when compared with those in the N group.The SCr and BUN in MO＋H/R group were much higher compared with those in the H/R group.The renal pathological changes were more severe in the H/R group than that of MO＋H/R group.Apoptosis phenomenon was lower in MO＋H/R group than H/R group（P〈0.05）.Conclusion Morphine postconditioning can protect rabbit against acute renal hypoxic/reoxygenation injury by reducing renal apoptosis.

关 键 词：吗啡后处理 缺氧/复氧损伤 细胞凋亡 

分 类 号：R965[医药卫生—药理学]